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Structural maintenance of chromosomes 4 is required for leukemia stem cell maintenance in MLL-AF9 induced acute myeloid leukemia.

Leuk. Lymphoma. 2018 Oct;59(10):2423-2430. doi:10.1080/10428194.2017.1387906. Epub 2017 Oct 18
Luyun Peng 1 , Yuanting Tang 1 , Yingchi Zhang 2 , Siqi Guo 1 , Leiwen Peng 1 , Lei Ye 1 , Yuefang Wang 1 , Yongmei Jiang 1
Luyun Peng 1 , Yuanting Tang 1 , Yingchi Zhang 2 , Siqi Guo 1 , Leiwen Peng 1 , Lei Ye 1 , Yuefang Wang 1 , Yongmei Jiang 1
+ et al

[No authors listed]

Author information
  • 1 b Key Laboratory of Birth Defects and Related Diseases of Women and Children, Sichuan University, Ministry of Education , Chengdu , China.
  • 2 c State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital , Chinese Academy of Medical Sciences and Peking Union Medical College , Tianjin , China.

摘要


The gene, structural maintenance of chromosomes 4 (SMC4) plays important role in chromosomes condensing and mitotic sister chromatid segregation, which has been revealed in regulating multiple cancer development and carcinogenesis. However, the role of SMC4 in acute myeloid leukemia (AML) propagation and its function in regulation of leukemia stem cells (LSCs) is not yet clear. Using an MLL-AF9 induced AML mouse model, we demonstrated that down modulating of SMC4 expression could prolong the survival time of AML mice. Furthermore, we found that knockdown SMC4 expression decreased the proportion of LSCs and affected its leukemia-initiating capacity. Cell cycle assay demonstrated that more LSCs were arrested in G0 phase by SMC4 knockdown. This activity was accompanied by increased expression of the Cdkn1a (P21) and Cdkn1b (P27) as well as decreased expression of CDK4. Therefore, our study revealed the critical role of SMC4 during AML progression and provided new insights into the mechanism of LSC maintenance.

KEYWORDS: AML, LSCs, MLL-AF9, SMC4, cell-cycle