Small Nuclear Ribonucleoprotein Polypeptide A-Mediated Alternative Polyadenylation of STAT5B during Th1 Cell Differentiation.

T cells are activated and differentiated into Th cells depending on the rapid and accurate changes in the cell transcriptome. In addition to changes in mRNA expression, the sequences of many transcripts are altered by alternative splicing and alternative polyadenylation (APA). We profiled the APA sites of human CD4+ T cell subsets with high-throughput sequencing and found that Th1 cells harbored more genes with shorter tandem 3' untranslated regions (UTRs) than did naive T cells. We observed that a key regulator of Th1 differentiation, possessed three major APA sites and preferred shorter in Th1 cells. In addition, small nuclear ribonucleoprotein polypeptide A (SNRPA) was found to bind directly to and facilitate its APA switching. We also found that p65 activation triggered by TCR signaling could promote SNRPA transcription and duanyu3 shortening of Thus we propose that the APA switching of duanyu18135B induced by TCR activation is mediated by SNRPA.

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