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Positive regulation of AMS by TDF1 and the formation of a TDF1-AMS complex are required for anther development in Arabidopsis thaliana.

New Phytol. 2018 Jan;217(1):378-391. doi:10.1111/nph.14790. Epub 2017 Sep 20
Yue Lou 1 , Hai-Sheng Zhou 1 , Yu Han 1 , Qiu-Ye Zeng 1 , Jun Zhu 1 , Zhong-Nan Yang 1
Yue Lou 1 , Hai-Sheng Zhou 1 , Yu Han 1 , Qiu-Ye Zeng 1 , Jun Zhu 1 , Zhong-Nan Yang 1
+ et al

[No authors listed]

Author information
  • 1 College of Life and Environment Sciences, Shanghai Normal University, 100 Guilin Road, Shanghai, 200234, China.

摘要


Tapetum development and pollen production are regulated by a complex transcriptional network that consists of a group of tapetum-specific Arabidopsis transcription factors (TFs). Among these TFs, DEFECTIVE IN TAPETAL DEVELOPMENT AND FUNCTION 1 (TDF1) encodes an R2R3 MYB factor, and ABORTED (AMS) encodes a basic helix-loop-helix (bHLH) factor. However, knowledge regarding the regulatory role of TDF1 in anther development remains limited. Here, we discovered that TDF1 directly regulates AMS via an AACCT cis-element. We found the precocious AMS transcript and absence of AMS protein in ams-/- gpTDF1:AMS-FLAG lines, suggesting the timing of the TDF1-regulated AMS expression is a prerequisite for AMS functioning. We found that TDF1 interacts with AMS. Additionally, the TDF1-AMS complex additively promotes the expression of AMS-regulated genes, suggesting that TDF1 and AMS regulate the downstream genes through a feed-forward loop. EPXB5, encoding a beta-expansin family protein, is another direct target of TDF1, and it is highly expressed in the tapetum and pollen grains. The TDF1-AMS complex acts in concert to activate EXPB5 expression through a feed-forward loop. The identification of the regulatory pathway between TDF1 and AMS provides an interlocked feed-forward loop circuit that precisely regulates the transcriptional cascades that support anther development. © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.

KEYWORDS: Arabidopsis thaliana , ABORTED MICROSPORE (AMS), EXPB5, TDF1, feed-forward loop, tapetum, transcriptional regulation