The replication and transcription activator of murine gammaherpesvirus 68 cooperatively enhances cytokine-activated, STAT3-mediated gene expression.

Gammaherpesviruses (γHVs) have a dynamic strategy for lifelong persistence, involving productive infection, latency, and intermittent reactivation. In latency reservoirs, such as B lymphocytes, γHVs exist as viral episomes and express few viral genes. Although the ability of γHV to reactivate from latency and re-enter the lytic phase is challenging to investigate and control, it is known that the γHV replication and transcription activator (RTA) can promote lytic reactivation. In this study, we provide first evidence that RTA of murine γΗV68 (MHV68) selectively binds and enhances the activity of tyrosine-phosphorylated host is a transcription factor classically activated by specific tyrosine 705 phosphorylation in response to cytokine stimulation. forms a dimer that avidly binds a consensus target site in the promoters of regulated genes, and our results indicate that RTA cooperatively enhances the ability of pTyr⁷⁰⁵-duanyu18133 to induce expression of a reporter gene. As indicated by coimmunoprecipitation, in latently infected B cells that are stimulated to reactivate MHV68, RTA bound specifically to endogenous An in vitro binding assay confirmed that RTA selectively recognizes pTyr⁷⁰⁵-duanyu18133 and indicated that the C-terminal transactivation domain of RTA was required for enhancing gene expression. The cooperation of these transcription factors may influence both viral and host genes. During MHV68 de novo infection, pTyr⁷⁰⁵-duanyu18133 promoted the temporal expression of ORF59, a viral replication protein. Our results demonstrate that MHV68 RTA specifically recognizes and recruits activated pTyr⁷⁰⁵-duanyu18133 during the lytic phase of infection.

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