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Role of rs13117307 single nuclear polymorphism in the risk of uterine cervical cancer from Polish population and its impact on exocyst complex component 1 expression.

Gene. 2017 Sep 05;627:524-529. Epub 2017 Jul 08
Sebastian Łaźniak 1 , Andrzej Roszak 2 , Adam Balcerek 1 , Żaneta Wareńczak-Florczak 3 , Edyta Prokop 4 , Anna Sowińska 5 , Emianka Sotiri 1 , Alexander Tsibulski 1 , Stefan Sajdak 6 , Pawel P Jagodziński 7
Sebastian Łaźniak 1 , Andrzej Roszak 2 , Adam Balcerek 1 , Żaneta Wareńczak-Florczak 3 , Edyta Prokop 4 , Anna Sowińska 5 , Emianka Sotiri 1 , Alexander Tsibulski 1 , Stefan Sajdak 6 , Pawel P Jagodziński 7
+ et al

[No authors listed]

Author information
  • 1 Department of Biochemistry and Molecular Biology, Poznań University of Medical Sciences, Poznań, Poland.
  • 2 Department of Radiotherapy and Gynecological Oncology, Greater Poland Cancer Center, Poznań, Poland; Department of Electroradiology, Poznań University of Medical Sciences, Poznań, Poland; Clinic of Gynecological Surgery, Poznań University of Medical Sciences, Poznań, Poland.
  • 3 Department of Radiotherapy and Gynecological Oncology, Greater Poland Cancer Center, Poznań, Poland; Department of Electroradiology, Poznań University of Medical Sciences, Poznań, Poland.
  • 4 I Clinic of Cardiology, Poznan University of Medical Sciences.
  • 5 Department of Computer Science and Statistics, Poznań University of Medical Sciences, Poznań, Poland.
  • 6 Clinic of Gynecological Surgery, Poznań University of Medical Sciences, Poznań, Poland.
  • 7 Department of Biochemistry and Molecular Biology, Poznań University of Medical Sciences, Poznań, Poland. Electronic address: pjagodzi@am.poznan.pl.

摘要


We evaluated the role of NM_001024924.1:c.1330+1646C>T (rs13117307) single nucleotide polymorphism (SNP), situated in the intronic region of exocyst complex component 1 (EXCO1), in the development and spreading of cervical squamous cell carcinoma (SCC). Utilizing high resolution melting curve analysis, we analyzed this polymorphism in patients with cervical SCC (n=485) and controls (n=509) in the Polish Caucasian population. Logistic regression analysis was used to adjust for age, parity, oral contraceptive use, tobacco smoking, and menopausal status. The influence of this polymorphism on the expression of EXCO1 was assessed by reverse transcription and real-time quantitative PCR analysis. For all patients with SCC, the p trend value calculated for rs13117307 was statistically significant (ptrend=0.0158). The adjusted odds ratio (OR) for T/T vs. C/C was 1.434 (95 % CI 1.105-1.861, p=0.007). We also found a significant contribution of rs13117307 to tumor stages III, IV and grade of differentiation G3. Other contributors are parity, oral contraceptive use, smoking, and women of postmenopausal age. We observed significant upregulation of EXCO1 transcript levels in the non-cancerous cervical tissues in carriers of the T/T vs. C/C (p=0.016), as well as an increase in the EXCO1 transcript levels in the cervical SCC tissue in carriers of the T/T vs. C/C (p=0.029) and for T/T vs C/T (p=0.0032). The rs13117307 SNP variants may upregulate the transcription of EXCO1, as well as the risk of development and spreading of cervical SCC.

KEYWORDS: Cervical cancer, Exocyst complex component 1, Expression, Polymorphism