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Proteomic Analysis of the E3 Ubiquitin-Ligase Hakai Highlights a Role in Plasticity of the Cytoskeleton Dynamics and in the Proteasome System.

J. Proteome Res.2017 Aug 04;16(8):2773-2788. doi:10.1021/acs.jproteome.7b00046. Epub 2017 Jul 21
Andrea Díaz-Díaz 1 , Alba Casas-Pais 1 , Valentina Calamia 2 , Raquel Castosa 1 , Olaia Martinez-Iglesias 1 , Daniel Roca-Lema 1 , Isabel Santamarina 1 , Manuel Valladares-Ayerbes 3 , Lourdes Calvo 4 , Venancio Chantada 1 , Angélica Figueroa 1
Andrea Díaz-Díaz 1 , Alba Casas-Pais 1 , Valentina Calamia 2 , Raquel Castosa 1 , Olaia Martinez-Iglesias 1 , Daniel Roca-Lema 1 , Isabel Santamarina 1 , Manuel Valladares-Ayerbes 3 , Lourdes Calvo 4 , Venancio Chantada 1 , Angélica Figueroa 1
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[No authors listed]

Author information
  • 1 Epithelial Plasticity and Metastasis Group, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas, Universidade da Coruña (UDC) , 15006 A Coruña, Spain.
  • 2 Proteomics Group-ProteoRed PRB2/ISCIII, INIBIC-CHUAC, UDC , 15006 A Coruña, Spain.
  • 3 Clinical and Translational Oncology Group, Hospital Universitario Virgen del Rocio, Instituto de Investigación Biomédica de Sevilla (IBIS) , 41013 Sevilla, Spain.
  • 4 Clinical and Translational Oncology Group, Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas , 15006 A Coruña, Spain.

摘要


Carcinoma, the most common type of cancer, arises from epithelial cells. The transition from adenoma to carcinoma is associated with the loss of E-cadherin and, in consequence, the disruption of cell-cell contacts. E-cadherin is a tumor suppressor, and it is down-regulated during epithelial-to-mesenchymal transition (EMT); indeed, its loss is a predictor of poor prognosis. Hakai is an E3 ubiquitin-ligase protein that mediates E-cadherin ubiquitination, endocytosis and finally degradation, leading the alterations of cell-cell contacts. Although E-cadherin is the most established substrate for Hakai activity, other regulated molecular targets for Hakai may be involved in cancer cell plasticity during tumor progression. In this work we employed an iTRAQ approach to explore novel molecular pathways involved in Hakai-driven EMT during tumor progression. Our results show that Hakai may have an important influence on cytoskeleton-related proteins, extracellular exosome-associated proteins, RNA-related proteins and proteins involved in metabolism. Moreover, a profound decreased expression in several proteasome subunits during Hakai-driven EMT was highlighted. Since proteasome inhibitors are becoming increasingly used in cancer treatment, our findings suggest that the E3 ubiquitin-ligase, such as Hakai, may be a better target than proteasome for using novel specific inhibitors in tumor subtypes that follow EMT.

KEYWORDS: E-cadherin, E3 ubiquitin-ligase, Hakai, epithelial-to-mesenchymal transition, proteasome