例如:"lncRNA", "apoptosis", "WRKY"

NHERF1 and NHERF2 regulation of SR-B1 stability via ubiquitination and proteasome degradation.

Biochem. Biophys. Res. Commun.2017 Sep 02;490(4):1168-1175. Epub 2017 Jun 29
Xiao Lu 1 , Lingfeng He 1 , Qian Zhou 1 , Meina Wang 1 , Wen-Jun Shen 2 , Salman Azhar 2 , Feiyan Pan 1 , Zhigang Guo 1 , Zhigang Hu 3
Xiao Lu 1 , Lingfeng He 1 , Qian Zhou 1 , Meina Wang 1 , Wen-Jun Shen 2 , Salman Azhar 2 , Feiyan Pan 1 , Zhigang Guo 1 , Zhigang Hu 3
+ et al

[No authors listed]

Author information
  • 1 Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, 1 WenYuan Road, Nanjing, 210023, China.
  • 2 Geriatric Research, Education and Clinical Center, VA Palo Alto Health Care System, Palo Alto, CA 94304, USA; Division of Endocrinology, Stanford University School of Medicine, Palo Alto, CA 94304, USA.
  • 3 Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, 1 WenYuan Road, Nanjing, 210023, China. Electronic address: huzg_2000@126.com.

摘要


Scavenger receptor class B type 1 (SR-B1), an HDL receptor plays a crucial role in cholesterol metabolism in the liver, steroidogenic tissues, and vascular cells including macrophages. SR-B1 is subject to regulation at the transcription, posttranscription and posttranslational levels. We previously provided evidence that PDZ domain containing NHERF1 and NHERF2 regulate SR-B1 protein levels post-transcriptionally, although the underlying mechanism(s) by which NHERF1 and NHERF2 regulate SR-B1 protein levels is not well understood. In this study, we demonstrate that SR-B1 is degraded intracellularly via ubiquitin-proteasome pathway and that SR-B1 can be ubiquitinated at K500 and K508 residues. Overexpression of NHERF1 or NHERF2 enhanced SR-B1 ubiquitination and degradation. NHERF1 and NHERF2 promote SR-B1 ubiquitination at sites K508 and K500, respectively. These results suggest that NHERF1 and NHERF2 down-regulated SR-B1 at least in part via the ubiquitin/proteasome pathway.

KEYWORDS: NHERF1, NHERF2, Protein stability, SR-B1, Ubiquitination