Adaptive optics improves multiphoton super-resolution imaging.
Nat Methods. 2017 Sep;14(9):869-872. Epub 2017 Jun 19
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摘要
We improve multiphoton structured illumination microscopy using a nonlinear guide star to determine optical aberrations and a deformable mirror to correct them. We demonstrate our method on bead phantoms, cells in collagen gels, nematode larvae and embryos, Drosophila brain, and zebrafish embryos. Peak intensity is increased (up to 40-fold) and resolution recovered (up to 176 ± 10 nm laterally, 729 ± 39 nm axially) at depths â¼250 μm from the coverslip surface.
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文献解读
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