JAK-STAT signaling regulation of chicken embryonic stem cell differentiation into male germ cells.

The Janus kinase-signal transducer and activator of transcription signaling is crucial in chicken germ stem cell differentiation, but its role in the regulation of germ cell differentiation is unknown. To address this, cucurbitacin I or interleukin 6 was used to inhibit or activate signaling during embryonic stem cells (ESCs) differentiation. The expression of downstream JAK-duanyu1813 signaling molecules was assessed by Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR). PAS, and immunohistochemical staining of frozen sections was used to determine the appearance of primordial germ cells (PGCs) and, later, spermatogonial stem cells (SSCs) during gonadal development. Inhibition of the JAK-duanyu1813 signaling resulted in decreased expression of JAK2 and as well as of PGCs markers; moreover, the proportion of CVH and C-KIT positive cells as well as the yield of PGCs were remarkably decreased, and the gonad was smaller than that of control samples. Conversely, activation of JAK-duanyu1813 resulted in increased expression of JAK2 and duanyu18133 as well as that of PGC marker CVH. In addition, the proportion of CVH and C-KIT-positive cells as well as the PGC yield was increased, and the gonad was significantly larger than that from control samples. Collectively, our results suggested that JAK-duanyu1813 effectively promoted the formation of PGCs in the genital ridge during early embryogenesis in vivo and played a positive role in the regulation of ESC to SSC differentiation in vitro, with JAK2 and duanyu18133 functioning as pivotal factors for intracellular signal transduction.

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