例如:"lncRNA", "apoptosis", "WRKY"

The Arabidopsis Cys2/His2 zinc finger transcription factor ZAT18 is a positive regulator of plant tolerance to drought stress.

J. Exp. Bot.2017 May 17;68(11):2991-3005
Mingzhu Yin 1 , Yanping Wang 2 , Lihua Zhang 3 , Jinzhu Li 1 , Wenli Quan 4 , Li Yang 1 , Qingfeng Wang 3 , Zhulong Chan 4
Mingzhu Yin 1 , Yanping Wang 2 , Lihua Zhang 3 , Jinzhu Li 1 , Wenli Quan 4 , Li Yang 1 , Qingfeng Wang 3 , Zhulong Chan 4
+ et al

[No authors listed]

Author information
  • 1 University of Chinese Academy of Sciences, Beijing 100039, China.
  • 2 Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
  • 3 Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden/Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, Hubei 430074, China.
  • 4 Key Laboratory for Quality Control of Characteristic Fruits and Vegetables of Hubei Province, College of Life Science and Technology, Hubei Engineering University, Xiaogan, Hubei 432000, China.

摘要


Environmental stress poses a global threat to plant growth and reproduction, especially drought stress. Zinc finger proteins comprise a family of transcription factors that play essential roles in response to various abiotic stresses. Here, we found that ZAT18 (At3g53600), a nuclear C2H2 zinc finger protein, was transcriptionally induced by dehydration stress. Overexpression (OE) of ZAT18 in Arabidopsis improved drought tolerance while mutation of ZAT18 resulted in decreased plant tolerance to drought stress. ZAT18 was preferentially expressed in stems, siliques, and vegetative rosette leaves. Subcellular location results revealed that ZAT18 protein was predominantly localized in the nucleus. ZAT18 OE plants exhibited less leaf water loss, lower content of reactive oxygen species higher leaf water content, and higher antioxidant enzyme activities after drought treatment when compared with the wild type (WT). RNA sequencing analysis showed that 423 and 561 genes were transcriptionally modulated by the ZAT18 transgene before and after drought treatment, respectively. Pathway enrichment analysis indicated that hormone metabolism, stress, and signaling were over-represented in ZAT18 OE lines. Several stress-responsive genes including COR47, ERD7, LEA6, and RAS1, and hormone signaling transduction-related genes including JAZ7 and PYL5 were identified as putative target genes of ZAT18. Taken together, ZAT18 functions as a positive regulator and plays a crucial role in the plant response to drought stress.

KEYWORDS: Drought stress, RNA sequencing, ZAT18, hormone, transcriptome analysis, zinc finger proteins

原始数据


 保存测序数据
Sample name
Organism Experiment title Sample type Library instrument Attributes
ecotypegenotypesource nametissuetreatment time
ZAT18 OE drought rep2
Arabidopsis thaliana GSM2466005: ZAT18 OE drought rep2; Arabidopsis thaliana; RNA-Seq RNA-Seq Illumina HiSeq 2000 Col-0 ZAT18 overexpression lines leaf leaf 10 days after drought
ZAT18 OE drought rep1
Arabidopsis thaliana GSM2466004: ZAT18 OE drought rep1; Arabidopsis thaliana; RNA-Seq RNA-Seq Illumina HiSeq 2000 Col-0 ZAT18 overexpression lines leaf leaf 10 days after drought
WT drought rep2
Arabidopsis thaliana GSM2466003: WT drought rep2; Arabidopsis thaliana; RNA-Seq RNA-Seq Illumina HiSeq 2000 Col-0 WT leaf leaf 10 days after drought
WT drought rep1
Arabidopsis thaliana GSM2466002: WT drought rep1; Arabidopsis thaliana; RNA-Seq RNA-Seq Illumina HiSeq 2000 Col-0 WT leaf leaf 10 days after drought
ZAT18 OE control rep2
Arabidopsis thaliana GSM2466001: ZAT18 OE control rep2; Arabidopsis thaliana; RNA-Seq RNA-Seq Illumina HiSeq 2000 Col-0 ZAT18 overexpression lines leaf leaf 10 days after drought