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Single-peptide DNA-dependent RNA polymerase homologous to multi-subunit RNA polymerase.

Nat Commun. 2017 Jun 06;8:15774
David Forrest 1 , Katherine James 1 , Yulia Yuzenkova 1 , Nikolay Zenkin 1
David Forrest 1 , Katherine James 1 , Yulia Yuzenkova 1 , Nikolay Zenkin 1

[No authors listed]

Author information
  • 1 Centre for Bacterial Cell Biology, Institute for Cell and Molecular Bioscience, Newcastle University, Baddiley-Clark Building, Richardson Road, Newcastle upon Tyne NE2 4AX, UK.

摘要


Transcription in all living organisms is accomplished by multi-subunit RNA polymerases (msRNAPs). msRNAPs are highly conserved in evolution and invariably share a ∼400 kDa five-subunit catalytic core. Here we characterize a hypothetical ∼100 kDa single-chain protein, YonO, encoded by the SPβ prophage of Bacillus subtilis. YonO shares very distant homology with msRNAPs, but no homology with single-subunit polymerases. We show that despite homology to only a few amino acids of msRNAP, and the absence of most of the conserved domains, YonO is a highly processive DNA-dependent RNA polymerase. We demonstrate that YonO is a bona fide RNAP of the SPβ bacteriophage that specifically transcribes its late genes, and thus represents a novel type of bacteriophage RNAPs. YonO and related proteins present in various bacteria and bacteriophages have diverged from msRNAPs before the Last Universal and, thus, may resemble the single-subunit ancestor of all msRNAPs.

原始数据


 保存测序数据
Sample name
Organism Experiment title Sample type Library instrument Attributes
genotypemitomycinsource namestrainsub species
ΔyonO mitomycin rep3
Bacillus subtilis subsp. subtilis str. 168 GSM2137067: ΔyonO mitomycin rep3; Bacillus subtilis subsp. subtilis str. 168; RNA-Seq RNA-Seq NextSeq 500 delta-yonO 0.5 ug/ml bacteria 168CA subtilis
ΔyonO mitomycin rep2
Bacillus subtilis subsp. subtilis str. 168 GSM2137066: ΔyonO mitomycin rep2; Bacillus subtilis subsp. subtilis str. 168; RNA-Seq RNA-Seq NextSeq 500 delta-yonO 0.5 ug/ml bacteria 168CA subtilis
ΔyonO mitomycin rep1
Bacillus subtilis subsp. subtilis str. 168 GSM2137065: ΔyonO mitomycin rep1; Bacillus subtilis subsp. subtilis str. 168; RNA-Seq RNA-Seq NextSeq 500 delta-yonO 0.5 ug/ml bacteria 168CA subtilis
ΔyonO rep3
Bacillus subtilis subsp. subtilis str. 168 GSM2137064: ΔyonO rep3; Bacillus subtilis subsp. subtilis str. 168; RNA-Seq RNA-Seq NextSeq 500 delta-yonO 0 ug/ml bacteria 168CA subtilis
ΔyonO rep2
Bacillus subtilis subsp. subtilis str. 168 GSM2137063: ΔyonO rep2; Bacillus subtilis subsp. subtilis str. 168; RNA-Seq RNA-Seq NextSeq 500 delta-yonO 0 ug/ml bacteria 168CA subtilis