A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells.

Elife. 2017 May 31;6. doi:10.7554/eLife.26420

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, encoding the Î³ subunit of the AMP Kinase complex. The Flip-Flop method is efficient and reliable, and permits conditional gene inactivation based on both spatial and temporal cues, in a cell cycle-, and developmental stage-independent fashion, creating a platform for systematic screens of gene function in developing and adult flies with unprecedented detail.

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A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells.

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