Genetic interaction mapping in mammalian cells using CRISPR interference.
Nat. Methods. 2017 Jun;14(6):577-580. Epub 2017 May 08
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摘要
We describe a combinatorial CRISPR interference (CRISPRi) screening platform for mapping genetic interactions in mammalian cells. We targeted 107 chromatin-regulation factors in human cells with pools of either single or double single guide RNAs (sgRNAs) to downregulate individual genes or gene pairs, respectively. Relative enrichment analysis of individual sgRNAs or sgRNA pairs allowed for quantitative characterization of genetic interactions, and comparison with protein-protein-interaction data revealed a functional map of chromatin regulation.
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基因功能
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原始数据
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文献解读
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