SPAG6 regulates cell apoptosis through the TRAIL signal pathway in myelodysplastic syndromes.

Myelodysplastic syndromes (MDSs) are a group of malignant clone hematopoietic stem-cell diseases, and the evolution and progression of MDS depend on the abnormal apoptosis of bone marrow cells. Our previous studies have indicated that sperm-associated located in the uniparental disomy regions of myeloid cells, is overexpressed in patients with MDS as compared to controls, and can inhibit apoptosis of SKM-1. However, the concrete mechanism is still unclear. In the present study, it was found that the TNF-related apoptosis-inducing ligand (TRAIL)signal pathway was activated when the expression of duanyu1842G6 was inhibited by lentivirus in SKM-1 cells. Additionally, the results of flow cytometry, Cell Counting Kit-8 assay and western blot analysis implied that the TRAIL signal pathway could be inhibited by a high expression of However, duanyu1842G6 cannot influence the expression of TRAIL death receptors, except for FADD. Additionally the interaction between FADD and TRAIL death receptors also increased in SKM-1 cells infected with duanyu1842G6-shRNA lentivirus. Thus, our study demonstrates that duanyu1842G6 may regulate apoptosis in SKM-1 through the TRAIL signal pathway, indicating that duanyu1842G6 could be a potential therapeutic target.

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