Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions.
Nat. Methods. 2017 Jun;14(6):573-576. Epub 2017 Mar 20
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摘要
We developed a systematic approach to map human genetic networks by combinatorial CRISPR-Cas9 perturbations coupled to robust analysis of growth kinetics. We targeted all pairs of 73 cancer genes with dual guide RNAs in three cell lines, comprising 141,912 tests of interaction. Numerous therapeutically relevant interactions were identified, and these patterns replicated with combinatorial drugs at 75% precision. From these results, we anticipate that cellular context will be critical to synthetic-lethal therapies.
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基因功能
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原始数据
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文献解读
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