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Two Triacylglycerol Pathway Genes, CTDNEP1 and LPIN1, are Down-Regulated by hsa-miR-122-5p in Hepatocytes.

Arch Iran Med. 2017 Mar;20(3):165-171
Mahmood Naderi 1 , Abdolreza Pazouki 2 , Ehsan Arefian 3 , Seyed Mahmoud Hashemi 4 , Fatemeh Jamshidi-Adegani 5 , Omid Gholamalamdari 5 , Sara Soudi 6 , Kayhan Azadmanesh 7 , Siamak Mirab Samiee 8 , Shahin Merat 1 , Mohammad Gholami Fesharaki 9 , Mahdieh Mondanizadeh 10 , Mohammad Vasei 11 , Masoud Soleimani 12
Mahmood Naderi 1 , Abdolreza Pazouki 2 , Ehsan Arefian 3 , Seyed Mahmoud Hashemi 4 , Fatemeh Jamshidi-Adegani 5 , Omid Gholamalamdari 5 , Sara Soudi 6 , Kayhan Azadmanesh 7 , Siamak Mirab Samiee 8 , Shahin Merat 1 , Mohammad Gholami Fesharaki 9 , Mahdieh Mondanizadeh 10 , Mohammad Vasei 11 , Masoud Soleimani 12
+ et al

[No authors listed]

Author information
  • 1 Liver and Pancreatobiliary Diseases Research Center, Digestive Disease Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
  • 2 Minimally Invasive Surgery Research Center, Iran University of Medical Sciences, Tehran, Iran.
  • 3 Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
  • 4 Department of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • 5 Stem Cell Technology Research Center, Tehran, Iran.
  • 6 Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
  • 7 Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
  • 8 Food and Drug Laboratory Research Center, Tehran, Iran.
  • 9 Department of Biostatistics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
  • 10 Department of Biotechnology and Molecular Medicine, Arak University of Medical Sciences, Arak, Iran.
  • 11 Liver and Pancreatobiliary Diseases Research Center, Digestive Disease Research Institute, Tehran University of Medical Sciences, Tehran, Iran, Department of Pathology, Tehran University of Medical Sciences, Shariati Hospital, Tehran, Iran.
  • 12 Department of Hematology, Tarbiat Modares University, Tehran, Iran.

摘要

BACKGROUND:Expression of miR-122 is highly specific to hepatocytes of the liver.  This miRNA is involved in lipid hemostasis of the tissue; however, there is no comprehensive understanding of its function in lipid hemostasis. MATERIALS AND METHODS:Since hepatocytes are responsible for part of Triacylglycerol (TAG) synthesis in the body, we hypothesized that miR-122, as the most abundant miRNA in the tissue, might regulate TAG metabolism by targeting key enzymes that are involved in its production pathway. A systematic computational analysis of putative targets of miR-122 identified CTDNEP1 and LPIN1 genes in the TAG pathway. We used dual-luciferase reporter assay, quantitative RT-PCR as well as western blot to confirm the repressive effect of miR-122 on CTDNEP1 and LPIN1 in TAG pathway. RESULTS:Real time PCR on liver needle biopsies with hepatosteatosis showed that miR-122 is up-regulated in hepatosteatosis. Surprisingly, the protein and RNA level of identified targets of miR-122 are also up-regulated in clinical samples, probably as a disproportionate feedback response to the high level of miR-122. CONCLUSION:Our findings suggest that up-regulation of miR-122 can trigger the compensatory response of LPIN1 and CTDNEP1 in hepatosteatosis.

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