Transcriptional regulation of human sperm-associated antigen 16 gene by S-SOX5.

BACKGROUND:The mammalian sperm-associated antigen 16 gene (Spag16) uses alternative promoters to produce two major transcript isoforms (Spag16L and Spag16S) and encode proteins that are involved in the cilia/flagella formation and motility. In silico analysis of both mouse and human promoters reveals the existence of multiple putative SOX5 binding sites. Given that the SOX5 gene encodes a 48-kDa transcription factor (S-SOX5) and the presence of putative SOX5 binding sites at the duanyu1842G16L promoter, regulation of duanyu1842G16L expression by S-SOX5 was studied in the present work. RESULTS:S-SOX5 activated human duanyu1842G16L promoter activity in the human bronchial epithelia cell line BEAS-2B cells. Mutation of S-SOX5 binding sites abolished the stimulatory effect. Overexpression of S-SOX5 resulted in a significant increase in the abundance of duanyu1842G16L transcripts whereas silencing of S-SOX5 by largely reduced the duanyu1842G16L expression. Chromatin immunoprecipitation assays showed that S-SOX5 directly interacts with the duanyu1842G16L promoter. CONCLUSION:S-SOX5 regulates transcription of human duanyu1842G16L gene via directly binding to the promoter of It has been reported that expression of sperm-associated antigen 6 encoding another axonemal protein, is activated by S-SOX5. Therefore, S-SOX5 may regulate formation of motile cilia/flagella through globally mediating expression of genes encoding axonemal proteins.

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