Development of a bimolecular luminescence complementation assay for RGS: G protein interactions in cells.
Anal. Biochem.2017 Apr 01;522:10-17. Epub 2017 Jan 20
Christopher R Bodle 1
,
Michael P Hayes 2
,
Joseph B O'Brien 3
,
David L Roman 4
Christopher R Bodle 1
,
Michael P Hayes 2
,
Joseph B O'Brien 3
,
David L Roman 4
+ et al
[No authors listed]
Author information
1 Department of Pharmaceutical Sciences and Experimental Therapeutics University of Iowa, 115 S. Grand Avenue S338 PHAR, Iowa City, IA, 52242, USA. Electronic address: christopher-bodle@uiowa.edu.
2 Department of Pharmaceutical Sciences and Experimental Therapeutics University of Iowa, 115 S. Grand Avenue S338 PHAR, Iowa City, IA, 52242, USA. Electronic address: michael-p-hayes@uiowa.edu.
3 Department of Pharmaceutical Sciences and Experimental Therapeutics University of Iowa, 115 S. Grand Avenue S338 PHAR, Iowa City, IA, 52242, USA. Electronic address: Joseph-obrien-1@uiowa.edu.
4 Department of Pharmaceutical Sciences and Experimental Therapeutics University of Iowa, 115 S. Grand Avenue S327 PHAR, Iowa City, IA, 52242, USA; Cancer Signaling and Experimental Therapeutics Program, Holden Comprehensive Cancer Center, University of Iowa Hospitals and Clinics, Iowa City, IA, USA. Electronic address: david-roman@uiowa.edu.
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摘要
protein-protein interaction. In conclusion, the NanoBit ProteinComplementationAssay holds promise as a robust platform for discovery and characterization of RGS inhibitors.
KEYWORDS: Gα(i1), Gα(q), High throughput screening, NanoBit, RGS