例如:"lncRNA", "apoptosis", "WRKY"

Protein Phosphatase 1 Down Regulates ZYG-1 Levels to Limit Centriole Duplication.

PLoS Genet.2017 Jan 19;13(1):e1006543. eCollection 2017 Jan
Nina Peel 1 , Jyoti Iyer 2 , Anar Naik 1 , Michael P Dougherty 2 , Markus Decker 3 , Kevin F O'Connell 2
Nina Peel 1 , Jyoti Iyer 2 , Anar Naik 1 , Michael P Dougherty 2 , Markus Decker 3 , Kevin F O'Connell 2
+ et al

[No authors listed]

Author information
  • 1 Department of Biology, The College of New Jersey, Ewing, NJ, United States of America.
  • 2 Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 8 Center Drive, Bethesda, MD, United States of America.
  • 3 Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
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摘要


In humans perturbations of centriole number are associated with tumorigenesis and microcephaly, therefore appropriate regulation of centriole duplication is critical. The C. elegans homolog of Plk4, ZYG-1, is required for centriole duplication, but our understanding of how ZYG-1 levels are regulated remains incomplete. We have identified the two PP1 orthologs, GSP-1 and GSP-2, and their regulators I-2SZY-2 and SDS-22 as key regulators of ZYG-1 protein levels. We find that down-regulation of PP1 activity either directly, or by mutation of szy-2 or sds-22 can rescue the loss of centriole duplication associated with a zyg-1 hypomorphic allele. Suppression is achieved through an increase in ZYG-1 levels, and our data indicate that PP1 normally regulates ZYG-1 through a post-translational mechanism. While moderate inhibition of PP1 activity can restore centriole duplication to a zyg-1 mutant, strong inhibition of PP1 in a wild-type background leads to centriole amplification via the production of more than one daughter centriole. Our results thus define a new pathway that limits the number of daughter centrioles produced each cycle.