[No authors listed]
The aim of the present study was to investigate whether urinary kidney injury moleculeâ1 (KIMâ1) presents a suitable early diagnostic biomarker of obstructive nephropathyâinduced acute kidney injury (AKI), and to develop a rapid detection method for urinary KIMâ1. Obstructive AKI was induced in an experimental rat model by a unilateral ureteral obstruction (UUO) operation. Macroâ and micromorphological kidney alterations were determined by visual observation and hematoxylin and eosin (HE) staining, respectively. Kidney functions were evaluated by detecting urea nitrogen and creatinine levels in rat urine and blood. Urinary KIMâ1 levels were measured using an enzymeâlinked immunosorbent assay, and the protein expression levels of KIMâ1, αâsmooth muscle actin (αâSMA) and vimentin in kidney tissues were detected using immunohistochemical assays. In order to measure KIMâ1 levels, colloidal gold immunochromatographic strips were developed based on the colloidal gold immunochromatographic assay. The results indicated that KIMâ1 levels were significantly higher in the UUO group when compared with the Sham group. KIMâ1 levels in the urine and kidney tissues exhibited a timeâdependent increase, together with increasing obstructive AKI in the UUO group. In addition, KIMâ1 levels were demonstrated to be a more sensitive biomarker of early obstructive AKI, when compared with αâSMA and vimentin. A colloidal goldâbased immunochromatographic strip was developed, whereby the detection of urinary KIMâ1 could be completed within 5â10 min. In conclusion, results of the present study demonstrated that urinary KIMâ1 may be a valuable biomarker for the early diagnosis of obstructive AKI, and the use of a colloidal gold immunochromatographic strip may be a promising method for the rapid detection of urinary KIMâ1.
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