例如:"lncRNA", "apoptosis", "WRKY"

Chronic Infection Depletes Hematopoietic Stem Cells through Stress-Induced Terminal Differentiation.

Cell Rep. 2016 Dec 06;17(10):2584-2595
Katie A Matatall 1 , Mira Jeong 2 , Siyi Chen 3 , Deqiang Sun 4 , Fengju Chen 5 , Qianxing Mo 5 , Marek Kimmel 6 , Katherine Y King 7
Katie A Matatall 1 , Mira Jeong 2 , Siyi Chen 3 , Deqiang Sun 4 , Fengju Chen 5 , Qianxing Mo 5 , Marek Kimmel 6 , Katherine Y King 7
+ et al

[No authors listed]

Author information
  • 1 Section of Pediatric Infectious Diseases, Baylor College of Medicine, Houston, TX 77030, USA; Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030, USA; Stem Cells and Regenerative Medicine Center, Baylor College of Medicine, Houston, TX 77030, USA.
  • 2 Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030, USA; Stem Cells and Regenerative Medicine Center, Baylor College of Medicine, Houston, TX 77030, USA.
  • 3 Department of Statistics, Rice University, Houston, TX 77030, USA.
  • 4 Institute of Biosciences & Technology, College of Medicine Texas A&M University Health Science Center, Houston, TX 77030, USA.
  • 5 Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, TX 77030, USA.
  • 6 Department of Statistics, Rice University, Houston, TX 77030, USA; Systems Engineering Group, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland.
  • 7 Section of Pediatric Infectious Diseases, Baylor College of Medicine, Houston, TX 77030, USA; Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030, USA; BCM Inflammation Center, Baylor College of Medicine, Houston, TX 77030, USA; Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, TX 77030, USA; Stem Cells and Regenerative Medicine Center, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address: kyk@bcm.edu.

摘要


Chronic infections affect a third of the world's population and can cause bone marrow suppression, a severe condition that increases mortality from infection. To uncover the basis for infection-associated bone marrow suppression, we conducted repeated infection of WT mice with Mycobacterium avium. After 4-6 months, mice became pancytopenic. Their hematopoietic stem and progenitor cells (HSPCs) were severely depleted and displayed interferon gamma (IFN-γ) signaling-dependent defects in self-renewal. There was no evidence of increased HSPC mobilization or apoptosis. However, consistent with known effects of IFN-γ, transcriptome analysis pointed toward increased myeloid differentiation of HSPCs and revealed the transcription factor Batf2 as a potential mediator of IFN-γ-induced HSPC differentiation. Gain- and loss-of-function studies uncovered a role for Batf2 in myeloid differentiation in both murine and human systems. We thus demonstrate that chronic infection can deplete HSPCs and identify BATF2 as a mediator of infection-induced HSPC terminal differentiation.

KEYWORDS: bone marrow failure, chronic infection, hematopoietic stem cell, interferon gamma, pancytopenia, terminal differentiation

原始数据


 保存测序数据
Sample name
Organism Experiment title Sample type Library instrument Attributes
agecell surface markercell typesexsource namestraintissuetreatment
ctr_hsc_l3 RNA-Seq
Mus musculus GSM2367465: ctr_hsc_l3 RNA-Seq; Mus musculus; RNA-Seq RNA-Seq Illumina HiSeq 2000 3-4 months SP KSL CD150+ Hematopoietic stem cells (HSCs) female Murine bone marrow derived primary HSCs C57BL/6 bone marrow Naïve
ctr_hsc_l2 RNA-Seq
Mus musculus GSM2367464: ctr_hsc_l2 RNA-Seq; Mus musculus; RNA-Seq RNA-Seq Illumina HiSeq 2000 5 months SP KSL CD150+ Hematopoietic stem cells (HSCs) female Murine bone marrow derived primary HSCs C57BL/6 bone marrow Naïve
ctr_hsc_l1 RNA-Seq
Mus musculus GSM2367463: ctr_hsc_l1 RNA-Seq; Mus musculus; RNA-Seq RNA-Seq Illumina HiSeq 2000 5 months SP KSL CD150+ Hematopoietic stem cells (HSCs) female Murine bone marrow derived primary HSCs C57BL/6 bone marrow Naïve
mav_hsc_l3 RNA-Seq
Mus musculus GSM2367462: mav_hsc_l3 RNA-Seq; Mus musculus; RNA-Seq RNA-Seq Illumina HiSeq 2000 3-4 months SP KSL CD150+ Hematopoietic stem cells (HSCs) female Murine bone marrow derived primary HSCs C57BL/6 bone marrow Mycobacterium avium infection
mav_hsc_l2 RNA-Seq
Mus musculus GSM2367461: mav_hsc_l2 RNA-Seq; Mus musculus; RNA-Seq RNA-Seq Illumina HiSeq 2000 5 months SP KSL CD150+ Hematopoietic stem cells (HSCs) female Murine bone marrow derived primary HSCs C57BL/6 bone marrow Mycobacterium avium infection