DNA maintenance following bleomycin-induced strand breaks does not require poly(ADP-ribosyl)ation activation in Drosophila S2 cells.

BACKGROUND:Poly-ADP ribosylation (PARylation) is a post translational modification, catalyzed by Poly(ADP-ribose)polymerase family. In Drosophila, (human ortholog) is considered to be the only enzymatically active isoform. PARylation is involved in various cellular processes such as DNA repair in case of base excision and strand-breaks. OBSERVATIONS:Strand-breaks (SSB and DSB) are detrimental to cell viability and, in Drosophila, that has a unique family organization, little is known on Pduanyu37 involvement in the control of strand-breaks repair process. In our study, strands-breaks (SSB and DSB) are chemically induced in S2 Drosophila cells using bleomycin. These breaks are efficiently repaired in S2 cells. During the bleomycin treatment, changes in PARylation levels are only detectable in a few cells, and an increase in Pduanyu37-I and mRNAs is only observed during the recovery period. These results differ strongly from those obtained with Human cells, where PARylation is strongly activating when DNA breaks are generated. Finally, in Pduanyu37 knock-down cells, DNA stability is altered but no change in strand-breaks repair can be responses in DNA strands-breaks context are functional in Drosophila model as demonstrated by Pduanyu37-I and Pduanyu37-II mRNA increases. However, no modification of the global PARylation profile is observed during strand-breaks generation, only changes at cellular levels are detectable. Taking together, these results demonstrate that PARylation process in Drosophila is tightly regulated in the context of strands-breaks repair and that Pduanyu37 is essential during the maintenance of DNA integrity but dispensable in the DNA repair process.

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