A Suite of Biochemical Assays for Screening RNA Methyltransferase BCDIN3D.
SLAS Discov. 2017 Jan;22(1):32-39. Epub 2016 Sep 27
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摘要
BCDIN3D is an RNA-methyltransferase that O-methylates the 5' phosphate of RNA and regulates microRNA maturation. To discover small-molecule inhibitors of BCDIN3D, a suite of biochemical assays was developed. A radiometric methyltransferase assay and fluorescence polarization-based S-adenosylmethionine and RNA displacement assays are described. In addition, differential scanning fluorimetry and surface plasmon resonance were used to characterize binding. These assays provide a comprehensive package for the development of small-molecule modulators of BCDIN3D activity.
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基因功能
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原始数据
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文献解读
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