[No authors listed]
In Escherichia coli, the expression of a Ï factor is expected to indirectly down-regulate the expression of genes recognized by another Ï factor, due to Ï factor competition for a limited pool of RNA polymerase core enzymes. Evidence suggests that the sensitivity of genes to indirect down-regulation differs widely. We studied the variability in this sensitivity in promoters primarily recognized by RNAP holoenzymes carrying Ï(70). From qPCR and live single-cell, single-RNA measurements of the transcription kinetics of several Ï(70)-dependent promoters in various conditions and from the analysis of Ï factors population-dependent models of transcription initiation, we find that, the smaller is the time-scale of the closed complex formation relative to the open complex formation, the weaker is a promoter's responsiveness to changes in Ï(38) numbers. We conclude that, in E. coli, a promoter's responsiveness to indirect regulation by Ï factor competition is determined by the sequence-dependent kinetics of the rate limiting steps of transcription initiation.
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