Expression of aquaporins 1 and 5 in a model of ventilator-induced lung injury and its relation to tidal volume.

GOALS:We analysed the expression of aquaporins 1 and 5 and its relation with tidal volume in a model of ventilator-induced lung injury. METHODS:42 rats were used. Six non-ventilated animals were sacrificed (group C). The remaining rats were ventilated for 2 hours with different tidal volumes (group 7 ML with 7 ml Kg(-1) , group 20ML with 20 ml kg(-1) ) and a respiratory rate of 90 bpm. Lung oedema was measured and the expression of AQP1 and AQP5 was determined by western immunoblotting and measurement of mRNA. RESULTS:Lung oedema and alveolar-capillary membrane permeability were significantly increased in the animals of group 20ML compared to the control group. AQP1 expression was decreased in groups 7ML and 20ML compared to group C. CONCLUSION:Mechanical ventilation with high tidal volume causes lung injury and oedema, increasing lung permeability. In ventilated rats with high tidal volume the pulmonary expression of AQP1 decreases. This article is protected by copyright. All rights reserved.

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