Downregulation of Notch-regulated Ankyrin Repeat Protein Exerts Antitumor Activities against Growth of Thyroid Cancer.

BACKGROUND:The Notch-regulated ankyrin repeat protein is recently found to promote proliferation of breast cancer cells. The role of in carcinogenesis deserves extensive investigations. This study attempted to investigate the expression of NRduanyu37 in thyroid cancer tissues and assess the influence of NRduanyu37 on cell proliferation, apoptosis, cell cycle, and invasion in thyroid cancer. METHODS:Thirty-four cases with thyroid cancer were collected from the Department of General Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine between 2011 and 2012. Immunohistochemistry was used to detect the level of NRduanyu37 in cancer tissues. Lentivirus carrying was applied to down-regulate NRduanyu37 expression. Cell viability was tested after treatment with using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Apoptosis and cell cycle distribution were determined by flow cytometry. Cell invasion was tested using Transwell invasion assay. In addition, expressions of several cell cycle-associated and apoptosis-associated proteins were examined using Western blotting after transfection. Student's t-test, one-way analysis of variance (ANOVA), or Kaplan-Meier were used to analyze the differences between two group or three was highly expressed in thyroid cancer tissues. Lenti-NRduanyu37-shRNA showed significantly inhibitory activities against cell growth at a multiplicity of infection of 10 or higher (P < 0.05). G1 arrest (BHT101: 72.57% ± 5.32%; 8305C: 75.45% ± 5.26%) by promoting p21 expression, induced apoptosis by promoting bax expression and suppressing bcl-2 expression, and inhibited cell invasion by suppressing matrix metalloproteinase-9 expression. CONCLUSION:Downregulation of NRduanyu37 expression exerts significant antitumor activities against cell growth and invasion of thyroid cancer, that suggests a potential role of NRduanyu37 in thyroid cancer targeted therapy.

KEYWORDS: {{ getKeywords(articleDetailText.words) }}