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Identification of potential biomarkers for post-traumatic complications released after trauma-hemorrhage from murine Kupffer cells and its investigation in lung and liver.

Biomarkers. 2016 Apr 27:1-8. doi:10.3109/1354750X.2016.1171908. Epub 2016 Apr 27
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摘要


CONTEXT:Early diagnosis of complications after severe trauma by specific biomarkers remains difficult. OBJECTIVE:Identify potential new biomarkers for early diagnosis of post-traumatic complications. MATERIAL AND METHODS:Mice underwent pressure-controlled hemorrhage or sham procedure. Four hours later, genome-wide expression of isolated Kupffer cells was compared with controls using Affymetrix-Genechip-Expression-Analysis and real-time-PCR. RESULTS:Expression analysis and real-time-PCR revealed a significant increase of gene expression of Cxcl10, Il4ra, Csf2rb2, Lcn2, and Gbp5. CONCLUSION:Cxcl10, Il4ra, Csf2rb2, Lcn2, and Gbp5 might represent new biomarkers for early diagnosis of post-traumatic complications, if they are linked to the development of post-traumatic complications.

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