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Delineation of metabolic gene clusters in plant genomes by chromatin signatures.

Nucleic Acids Res.2016 Mar 18;44(5):2255-65. Epub 2016 Feb 18
Nan Yu 1 , Hans-Wilhelm Nützmann 2 , James T MacDonald 3 , Ben Moore 3 , Ben Field 1 , Souha Berriri 1 , Martin Trick 1 , Susan J Rosser 4 , S Vinod Kumar 1 , Paul S Freemont 3 , Anne Osbourn 5
Nan Yu 1 , Hans-Wilhelm Nützmann 2 , James T MacDonald 3 , Ben Moore 3 , Ben Field 1 , Souha Berriri 1 , Martin Trick 1 , Susan J Rosser 4 , S Vinod Kumar 1 , Paul S Freemont 3 , Anne Osbourn 5
+ et al

[No authors listed]

Author information
  • 1 John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
  • 2 John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK hans-wilhelm.nutzmann@jic.ac.uk.
  • 3 Centre for Synthetic Biology and Innovation, Imperial College, South Kensington Campus, London, SW7 2AZ, UK.
  • 4 School of Biological Sciences, University of Edinburgh, King's Building, Edinburgh, EH9 3JR, UK.
  • 5 John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK anne.osbourn@jic.ac.uk.

摘要


Plants are a tremendous source of diverse chemicals, including many natural product-derived drugs. It has recently become apparent that the genes for the biosynthesis of numerous different types of plant natural products are organized as metabolic gene clusters, thereby unveiling a highly unusual form of plant genome architecture and offering novel avenues for discovery and exploitation of plant specialized metabolism. Here we show that these clustered pathways are characterized by distinct chromatin signatures of histone 3 lysine trimethylation (H3K27me3) and histone 2 variant H2A.Z, associated with cluster repression and activation, respectively, and represent discrete windows of co-regulation in the genome. We further demonstrate that knowledge of these chromatin signatures along with chromatin mutants can be used to mine genomes for cluster discovery. The roles of H3K27me3 and H2A.Z in repression and activation of single genes in plants are well known. However, our discovery of highly localized operon-like co-regulated regions of chromatin modification is unprecedented in plants. Our findings raise intriguing parallels with groups of physically linked multi-gene complexes in animals and with clustered pathways for specialized metabolism in filamentous fungi. © The Author(s) 2016. Published by Oxford University Press on behalf of Research.