siRNA-TMEM98 inhibits the invasion and migration of lung cancer cells.

Non-small cell lung cancer (NSCLC) is one of the most common human malignancies, which threatens people's life heavily. Recently, TMEM98 is supposed to be of great value for the discoveries of anti-tumor drugs. We attempt to explore the biological role of TMEM98 in the human lung carcinoma. Clinical lung cancer tissue and normal tissue were collected, and the mRNA expression of TMEM98 in cancer tissue was significantly higher than that in normal tissue. By real-time-PCR and Western blot analysis of TMEM98 expression, human A549 and H460 cells were determined to carry out further investigations. By CCK8, it is found that siRNA-TMEM98 treatment effectively suppressed the proliferation of A549 and H460 cells. In addition, the invasion and migration of A549 and H460 cells were also inhibited by siRNA-TMEM98. We then studied the invasion and migration related proteins level by Western blot. From our result, the protein expression of MMP-2, MMP-9, RhoC and MTA1 were all regulated dramatically in siRNA-TMEM98 groups compared with the control and mock group. To conclude, our results indicated that siRNA-TMEM98 inhibited the invasion and migration of lung cancer cells, which can be considered as a novel target for NSCLC treatment.

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