例如:"lncRNA", "apoptosis", "WRKY"

Comparative transcriptomic profiling of hydrogen peroxide signaling networks in zebrafish and human keratinocytes: Implications toward conservation, migration and wound healing.

Sci Rep. 2016 Feb 05;6:20328
Thomas S Lisse 1 , Benjamin L King 2 , Sandra Rieger 2
Thomas S Lisse 1 , Benjamin L King 2 , Sandra Rieger 2

[No authors listed]

Author information
  • 1 The Jackson Laboratory, Bar Harbor, Maine 04609, U.S.A.
  • 2 Davis Center for Regenerative Biology and Medicine, MDI Biological Laboratory, Kathryn W. Davis Building 227, Old Bar Harbor Road, Salisbury Cove, Maine 04672, U.S.A.
全文

摘要


Skin wounds need to be repaired rapidly after injury to restore proper skin barrier function. Hydrogen peroxide (H2O2) is a conserved signaling factor that has been shown to promote a variety of skin wound repair processes, including immune cell migration, angiogenesis and sensory axon repair. Despite growing research on H2O2 functions in wound repair, the downstream signaling pathways activated by this reactive oxygen species in the context of injury remain largely unknown. The goal of this study was to provide a comprehensive analysis of gene expression changes in the epidermis upon exposure to H2O2 concentrations known to promote wound repair. Comparative transcriptome analysis using RNA-seq data from larval zebrafish and previously reported microarray data from a human epidermal keratinocyte line shows that H2O2 activates conserved cell migration, adhesion, cytoprotective and anti-apoptotic programs in both zebrafish and human keratinocytes. Further assessment of expression characteristics and signaling pathways revealed the activation of three major H2O2-dependent pathways, EGF, FOXO1, and IKKα. This study expands on our current understanding of the clinical potential of low-level H2O2 for the promotion of epidermal wound repair and provides potential candidates in the treatment of wound healing deficits.

原始数据


 保存测序数据
Sample name
Organism Experiment title Sample type Library instrument Attributes
agesource namestraintissue
H202 treated 3_RNAseq
Danio rerio GSM1964821: H202 treated 3_RNAseq; Danio rerio; RNA-Seq RNA-Seq Illumina HiSeq 2000 3dpf whole larvae_H2O2 Nacre whole larvae
H202 treated 2_RNAseq
Danio rerio GSM1964820: H202 treated 2_RNAseq; Danio rerio; RNA-Seq RNA-Seq Illumina HiSeq 2000 3dpf whole larvae_H2O2 Nacre whole larvae
H202 treated 1_RNAseq
Danio rerio GSM1964819: H202 treated 1_RNAseq; Danio rerio; RNA-Seq RNA-Seq Illumina HiSeq 2000 3dpf whole larvae_H2O2 Nacre whole larvae
Untreated 3_RNAseq
Danio rerio GSM1964818: Untreated 3_RNAseq; Danio rerio; RNA-Seq RNA-Seq Illumina HiSeq 2000 3dpf whole larvae_untreated Nacre whole larvae
Untreated 2_RNAseq
Danio rerio GSM1964817: Untreated 2_RNAseq; Danio rerio; RNA-Seq RNA-Seq Illumina HiSeq 2000 3dpf whole larvae_untreated Nacre whole larvae