Skin wounds need to be repaired rapidly after injury to restore proper skin barrier function. Hydrogen peroxide (H2O2) is a conserved signaling factor that has been shown to promote a variety of skin wound repair processes, including immune cell migration, angiogenesis and sensory axon repair. Despite growing research on H2O2 functions in wound repair, the downstream signaling pathways activated by this reactive oxygen species in the context of injury remain largely unknown. The goal of this study was to provide a comprehensive analysis of gene expression changes in the epidermis upon exposure to H2O2 concentrations known to promote wound repair. Comparative transcriptome analysis using RNA-seq data from larval zebrafish and previously reported microarray data from a human epidermal keratinocyte line shows that H2O2 activates conserved cell migration, adhesion, cytoprotective and anti-apoptotic programs in both zebrafish and human keratinocytes. Further assessment of expression characteristics and signaling pathways revealed the activation of three major H2O2-dependent pathways, EGF, FOXO1, and IKKα. This study expands on our current understanding of the clinical potential of low-level H2O2 for the promotion of epidermal wound repair and provides potential candidates in the treatment of wound healing deficits.
eevs, cyp24a1, vsig10, il6st, si:ch211-210b2.2, odc1, cyp1a, epha2a, itgb4, junbb, mc1r, ftr53, scd, mmp13a, mmp9, sqstm1, nfkbiaa, socs3b, junba, gadd45bb, xpc, itga11a, tnfb, mknk1, hsp70l, si:ch211-132f19.7, elf3, klf4, itpkb, fosl1a, arhgap27l, plek2, ctdspl3, si:dkey-46i9.6, npas4a, cnot7, foxo1a, hmox1a, egr4
Sample name | Organism | Experiment title | Sample type | Library instrument | Attributes | |||||||||||||||||||||||||||||||||||||||||||||||||
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age | source name | strain | tissue | |||||||||||||||||||||||||||||||||||||||||||||||||||
H202 treated 3_RNAseq | Danio rerio | GSM1964821: H202 treated 3_RNAseq; Danio rerio; RNA-Seq | RNA-Seq | Illumina HiSeq 2000 | 3dpf | whole larvae_H2O2 | Nacre | whole larvae | ||||||||||||||||||||||||||||||||||||||||||||||
H202 treated 2_RNAseq | Danio rerio | GSM1964820: H202 treated 2_RNAseq; Danio rerio; RNA-Seq | RNA-Seq | Illumina HiSeq 2000 | 3dpf | whole larvae_H2O2 | Nacre | whole larvae | ||||||||||||||||||||||||||||||||||||||||||||||
H202 treated 1_RNAseq | Danio rerio | GSM1964819: H202 treated 1_RNAseq; Danio rerio; RNA-Seq | RNA-Seq | Illumina HiSeq 2000 | 3dpf | whole larvae_H2O2 | Nacre | whole larvae | ||||||||||||||||||||||||||||||||||||||||||||||
Untreated 3_RNAseq | Danio rerio | GSM1964818: Untreated 3_RNAseq; Danio rerio; RNA-Seq | RNA-Seq | Illumina HiSeq 2000 | 3dpf | whole larvae_untreated | Nacre | whole larvae | ||||||||||||||||||||||||||||||||||||||||||||||
Untreated 2_RNAseq | Danio rerio | GSM1964817: Untreated 2_RNAseq; Danio rerio; RNA-Seq | RNA-Seq | Illumina HiSeq 2000 | 3dpf | whole larvae_untreated | Nacre | whole larvae |