Rapid Changes in the Translatome during the Conversion of Growth Cones to Synaptic Terminals.

Cell Rep. 2016 Feb 09;14(5):1258-1271. Epub 2016 Jan 28

Kelvin Xi Zhang ¹ , Liming Tan ¹ , Matteo Pellegrini ² , S Lawrence Zipursky ³ , Jason M McEwen ¹

Author information

¹ Department of Biological Chemistry, Howard Hughes Medical Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles CA 90095, USA.
² Department of Molecular, Cell and Developmental Biology, University of California Los Angeles, P.O. Box 951606, Los Angeles, CA 90095, USA.
³ Department of Biological Chemistry, Howard Hughes Medical Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles CA 90095, USA. Electronic address: lzipursky@mednet.ucla.edu.

摘要

A common step in the formation of neural circuits is the conversion of growth cones to presynaptic terminals. Characterizing patterns of global gene expression during this process is problematic due to the cellular diversity of the brain and the complex temporal dynamics of development. Here, we take advantage of the synchronous conversion of Drosophila photoreceptor growth cones into presynaptic terminals to explore global changes in gene expression during presynaptic differentiation. Using a tandemly tagged ribosome trap (T-TRAP) and RNA sequencing (RNA-seq) at multiple developmental times, we observed dramatic changes in coding and non-coding RNAs with presynaptic differentiation. Marked changes in the mRNA encoding transmembrane and secreted proteins occurred preferentially. The of transcripts encoding synaptic proteins were preferentially lengthened, and these extended UTRs were preferentially enriched for sites recognized by RNA binding proteins. These data provide a rich resource for uncovering the regulatory logic underlying presynaptic differentiation.

原始数据

保存测序数据

Sample name	Organism	Experiment title	Sample type	Library instrument	Attributes
Sample name	Organism	Experiment title	Sample type	Library instrument	age	cell type	genotype	source name	tissue
96hrs APF rep2	Drosophila melanogaster	GSM1377063: 96hrs APF rep2; Drosophila melanogaster; RNA-Seq	RNA-Seq	Illumina HiSeq 2000	96 hrs APF	Photoreceptor neurons	wild type	Photoreceptor neurons	retina
96hrs APF rep1	Drosophila melanogaster	GSM1377062: 96hrs APF rep1; Drosophila melanogaster; RNA-Seq	RNA-Seq	Illumina HiSeq 2000	96 hrs APF	Photoreceptor neurons	wild type	Photoreceptor neurons	retina
65hrs APF rep2	Drosophila melanogaster	GSM1377061: 65hrs APF rep2; Drosophila melanogaster; RNA-Seq	RNA-Seq	Illumina HiSeq 2000	65 hrs APF	Photoreceptor neurons	wild type	Photoreceptor neurons	retina
65hrs APF rep1	Drosophila melanogaster	GSM1377060: 65hrs APF rep1; Drosophila melanogaster; RNA-Seq	RNA-Seq	Illumina HiSeq 2000	65 hrs APF	Photoreceptor neurons	wild type	Photoreceptor neurons	retina
53hrs APF rep2	Drosophila melanogaster	GSM1377059: 53hrs APF rep2; Drosophila melanogaster; RNA-Seq	RNA-Seq	Illumina HiSeq 2000	53 hrs APF	Photoreceptor neurons	wild type	Photoreceptor neurons	retina