例如:"lncRNA", "apoptosis", "WRKY"

Galectin expression in healing wounded skin treated with low-temperature plasma: Comparison with treatment by electronical coagulation.

Arch. Biochem. Biophys.2016 Jan 28. Epub 2016 Jan 28
Yoshihiro Akimoto 1 , Sanae Ikehara 2 , Takashi Yamaguchi 2 , Jaeho Kim 3 , Hayato Kawakami 4 , Nobuyuki Shimizu 5 , Masaru Hori 6 , Hajime Sakakita 3 , Yuzuru Ikehara 7
Yoshihiro Akimoto 1 , Sanae Ikehara 2 , Takashi Yamaguchi 2 , Jaeho Kim 3 , Hayato Kawakami 4 , Nobuyuki Shimizu 5 , Masaru Hori 6 , Hajime Sakakita 3 , Yuzuru Ikehara 7
+ et al

[No authors listed]

Author information
  • 1 Department of Anatomy, Kyorin University School of Medicine, Mitaka, 181-8611, Japan. Electronic address: yakimoto@ks.kyorin-u.ac.jp.
  • 2 Biotechnology Research Institute for Drug Discovery, The National Institutes of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.
  • 3 Electronics and Photonics Research Institute, AIST, Tsukuba Central 2, 1-1-1 Umezono, Tsukuba, Ibaraki, 305-8568, Japan.
  • 4 Department of Anatomy, Kyorin University School of Medicine, Mitaka, 181-8611, Japan.
  • 5 International University of Health and Welfare, Sanno Hospital, Akasaka, 107-0052, Japan.
  • 6 Graduate School of Engineering, Nagoya University, Nagoya, 464-8603, Japan.
  • 7 Biotechnology Research Institute for Drug Discovery, The National Institutes of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan; Graduate School of Medicine, Chiba University, Chiba, 107-0052, Japan.

摘要


Low-temperature plasma is useful for the care of wounded skin. It accelerates wound healing. However, the mechanism of this effect has not been fully elucidated yet. Galectin-1 is reported to accelerate wound healing via the Smad signaling pathway. In the present study to clarify whether or not galectins were expressed during the process of wound healing in the plasma-treated skin, we examined the effect of low-temperature plasma on galectin expression in the healing skin. We compared the effects of low-temperature plasma on the expression of galectin-1, -2, and -3 in the healing skin with those of electrocoagulation conducted with a high-frequency electrical coagulator. Immediately after the start of low-temperature plasma treatment following the incision made in the skin, a membrane-like structure was formed on the surface of the wound. Immunoelectron microscopy showed that these galectins were localized in the membrane-like structure of the plasma-treated skin. The expressions of these galectins were increased by the low-temperature plasma treatment, whereas they were inhibited by the electrocoagulation. These results suggest that galectins were involved in the wound healing of low-temperature plasma-treated skin. Galectins will thus be good markers for further examination of the effects of low-temperature plasma on the healing of wounded skin.

KEYWORDS: Electrocoagulation, Galectin, Immunohistochemistry, Low-temperature plasma, Skin, Wound healing