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Functional Interplay of Two Paralogs Encoding SWI/SNF Chromatin-Remodeling Accessory Subunits During Caenorhabditis elegans Development.

Genetics. 2016 Mar;202(3):961-75. Epub 2016 Jan 06
Iris Ertl 1 , Montserrat Porta-de-la-Riva 2 , Eva Gómez-Orte 3 , Karinna Rubio-Peña 1 , David Aristizábal-Corrales 1 , Eric Cornes 1 , Laura Fontrodona 1 , Xabier Osteikoetxea 1 , Cristina Ayuso 4 , Peter Askjaer 4 , Juan Cabello 3 , Julián Cerón 5
Iris Ertl 1 , Montserrat Porta-de-la-Riva 2 , Eva Gómez-Orte 3 , Karinna Rubio-Peña 1 , David Aristizábal-Corrales 1 , Eric Cornes 1 , Laura Fontrodona 1 , Xabier Osteikoetxea 1 , Cristina Ayuso 4 , Peter Askjaer 4 , Juan Cabello 3 , Julián Cerón 5
+ et al

[No authors listed]

Author information
  • 1 Cancer and Human Molecular Genetics, Bellvitge Biomedical Research Institute-IDIBELL, 08908 L'Hospitalet de Llobregat, Barcelona, Spain.
  • 2 Cancer and Human Molecular Genetics, Bellvitge Biomedical Research Institute-IDIBELL, 08908 L'Hospitalet de Llobregat, Barcelona, Spain C. elegans Core Facility, Bellvitge Biomedical Research Institute-IDIBELL, L'Hospitalet de Llobregat, Barcelona, Spain.
  • 3 Center for Biomedical Research of La Rioja (CIBIR), 26006 Logroño, Spain.
  • 4 Andalusian Center for Developmental Biology (CABD), Consejo Superior de Investigaciones Científicas/Junta de Andalucia/Universidad Pablo de Olavide, 41013 Seville, Spain.
  • 5 Cancer and Human Molecular Genetics, Bellvitge Biomedical Research Institute-IDIBELL, 08908 L'Hospitalet de Llobregat, Barcelona, Spain jceron@idibell.cat.

摘要


SWI/SNF ATP-dependent chromatin-remodeling complexes have been related to several cellular processes such as transcription, regulation of chromosomal stability, and DNA repair. The Caenorhabditis elegans gene ham-3 (also known as swsn-2.1) and its paralog swsn-2.2 encode accessory subunits of SWI/SNF complexes. Using RNA interference assays and diverse alleles we investigated whether ham-3 and swsn-2.2 have different functions during C. elegans development since they encode proteins that are probably mutually exclusive in a given SWI/SNF complex. We found that ham-3 and swsn-2.2 display similar functions in vulva specification, germline development, and intestinal cell proliferation, but have distinct roles in embryonic development. Accordingly, we detected functional redundancy in some developmental processes and demonstrated by RNA sequencing of L4 animals that ham-3 and swsn-2.2 regulate the expression of a common subset of genes but also have specific targets. Cell lineage analyses in the embryo revealed hyper-proliferation of intestinal cells in ham-3 null mutants whereas swsn-2.2 is required for proper cell divisions. Using a proteomic approach, we identified SWSN-2.2-interacting proteins needed for early cell divisions, such as SAO-1 and ATX-2, and also nuclear envelope proteins such as MEL-28. swsn-2.2 mutants phenocopy mel-28 loss-of-function, and we observed that SWSN-2.2 and MEL-28 colocalize in mitotic and meiotic chromosomes. Moreover, we demonstrated that SWSN-2.2 is required for correct chromosome segregation and nuclear reassembly after mitosis including recruitment of MEL-28 to the nuclear periphery.

KEYWORDS: Caenorhabditis elegans, SWI/SNF, chromatin, development, nuclear envelope

原始数据


 保存测序数据
Sample name
Organism Experiment title Sample type Library instrument Attributes
development stagesource namestrain
swsn-2.2(RNAi) N2 L4
Caenorhabditis elegans GSM1964359: swsn-2.2(RNAi) N2 L4; Caenorhabditis elegans; RNA-Seq RNA-Seq Illumina HiSeq 2000 Larva 4 wild type N2 N2
ham-3(RNAi) N2 L4
Caenorhabditis elegans GSM1964358: ham-3(RNAi) N2 L4; Caenorhabditis elegans; RNA-Seq RNA-Seq Illumina HiSeq 2000 Larva 4 wild type N2 N2
gfp(RNAi) N2 L4
Caenorhabditis elegans GSM1964357: gfp(RNAi) N2 L4; Caenorhabditis elegans; RNA-Seq RNA-Seq Illumina HiSeq 2000 Larva 4 wild type N2 N2