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The mitogen-activated protein kinase phosphatase PHS1 regulates flowering in Arabidopsis thaliana.

Planta. 2016 Apr;243(4):909-23. Epub 2015 Dec 31
Qian Tang 1 , Emilie Guittard-Crilat 2 , Régis Maldiney 2 , Yvette Habricot 3 , Emile Miginiac 2 , Jean-Pierre Bouly 4 , Sandrine Lebreton 2
Qian Tang 1 , Emilie Guittard-Crilat 2 , Régis Maldiney 2 , Yvette Habricot 3 , Emile Miginiac 2 , Jean-Pierre Bouly 4 , Sandrine Lebreton 2
+ et al

[No authors listed]

Author information
  • 1 Plant Biological Sciences Graduate Program, Department of Horticultural Science, Microbial and Plant Genomics Institute, University of Minnesota, 1970 Folwell Avenue, Saint Paul, MN, 55108, USA.
  • 2 Adaptation des Plantes aux Contraintes Environnementales, Sorbonne Universités, UPMC Univ Paris 06, URF5, 75005, Paris, France.
  • 3 Biologie du Développement, CNRS, UMR 7622, 75005, Paris, France.
  • 4 Computational and Quantitative Biology, CNRS-UPMC UMR 7238, 15, rue de l'Ecole de Médecine, 75006, Paris, France. jean-pierre.bouly@upmc.fr.

摘要


MAIN CONCLUSION:Arabidopsis PHS1, initially known as an actor of cytoskeleton organization, is a positive regulator of flowering in the photoperiodic and autonomous pathways by modulating both CO and FLC mRNA levels. Protein phosphorylation and dephosphorylation is a major type of post-translational modification, controlling many biological processes. In Arabidopsis thaliana, five genes encoding MAPK phosphatases (MKP)-like proteins have been identified. Among them, PROPYZAMIDE HYPERSENSITIVE 1 (PHS1) encoding a dual-specificity protein tyrosine phosphatase (DsPTP) has been shown to be involved in microtubule organization, germination and ABA-regulated stomatal opening. Here, we demonstrate that PHS1 also regulates flowering under long-day and short-day conditions. Using physiological, genetic and molecular approaches, we have shown that the late flowering phenotype of the knock-out phs1-5 mutant is linked to a higher expression of FLOWERING LOCUS C (FLC). In contrast, a decline of both CONSTANS (CO) and FLOWERING LOCUS T (FT) expression is observed in the knock-out phs1-5 mutant, especially at the end of the light period under long-day conditions when the induction of flowering occurs. We show that this partial loss of sensitivity to photoperiodic induction is independent of FLC. Our results thus indicate that PHS1 plays a dual role in flowering, in the photoperiodic and autonomous pathways, by modulating both CO and FLC mRNA levels. Our work reveals a novel actor in the complex network of the flowering regulation.

KEYWORDS: Arabidopsis, CONSTANS, FLOWERING LOCUS C (FLC), FLOWERING LOCUS T (FT), Flowering, Phosphatase