例如:"lncRNA", "apoptosis", "WRKY"

Casein kinase II promotes target silencing by miRISC through direct phosphorylation of the DEAD-box RNA helicase CGH-1.

Proc. Natl. Acad. Sci. U.S.A.2015 Dec 29;112(52):E7213-22. Epub 2015 Dec 15
Amelia F Alessi 1 , Vishal Khivansara 2 , Ting Han 2 , Mallory A Freeberg 3 , James J Moresco 4 , Patricia G Tu 4 , Eric Montoye 5 , John R Yates 4 , Xantha Karp 5 , John K Kim 6
Amelia F Alessi 1 , Vishal Khivansara 2 , Ting Han 2 , Mallory A Freeberg 3 , James J Moresco 4 , Patricia G Tu 4 , Eric Montoye 5 , John R Yates 4 , Xantha Karp 5 , John K Kim 6
+ et al

[No authors listed]

Author information
  • 1 Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109; Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109;
  • 2 Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109;
  • 3 Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109; Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI 48109;
  • 4 Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037;
  • 5 Department of Biology, Central Michigan University, Mount Pleasant, MI 48859.
  • 6 Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109; Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109; jnkim@jhu.edu.

摘要


MicroRNAs (miRNAs) play essential, conserved roles in diverse developmental processes through association with the miRNA-induced silencing complex (miRISC). Whereas fundamental insights into the mechanistic framework of miRNA biogenesis and target gene silencing have been established, posttranslational modifications that affect miRISC function are less well understood. Here we report that the conserved serine/threonine kinase, casein kinase II (CK2), promotes miRISC function in Caenorhabditis elegans. CK2 inactivation results in developmental defects that phenocopy loss of miRISC cofactors and enhances the loss of miRNA function in diverse cellular contexts. Whereas CK2 is dispensable for miRNA biogenesis and the stability of miRISC cofactors, it is required for efficient miRISC target mRNA binding and silencing. Importantly, we identify the conserved DEAD-box RNA helicase, CGH-1/DDX6, as a key CK2 substrate within miRISC and demonstrate phosphorylation of a conserved N-terminal serine is required for CGH-1 function in the miRNA pathway.

KEYWORDS: CGH-1, casein kinase II, miRISC, microRNA, phosphorylation

原始数据


 保存测序数据
Sample name
Organism Experiment title Sample type Library instrument Attributes
RNAi treatmentStagesource namestrain
Small RNAs in WT C. elegans under alg-1 RNAi
Caenorhabditis elegans GSM1631684: Small RNAs in WT C. elegans under alg-1 RNAi; Caenorhabditis elegans; miRNA-Seq miRNA-Seq Illumina HiSeq 2000 alg-1 L4 Whole animal N2
Small RNAs in WT C. elegans under kin-10 RNAi
Caenorhabditis elegans GSM1631683: Small RNAs in WT C. elegans under kin-10 RNAi; Caenorhabditis elegans; miRNA-Seq miRNA-Seq Illumina HiSeq 2000 kin-10 L4 Whole animal N2
Small RNAs in WT C. elegans under kin-3 RNAi
Caenorhabditis elegans GSM1631682: Small RNAs in WT C. elegans under kin-3 RNAi; Caenorhabditis elegans; miRNA-Seq miRNA-Seq Illumina HiSeq 2000 kin-3 L4 Whole animal N2
Small RNAs in WT C. elegans under L4440 RNAi
Caenorhabditis elegans GSM1631681: Small RNAs in WT C. elegans under L4440 RNAi; Caenorhabditis elegans; miRNA-Seq miRNA-Seq Illumina HiSeq 2000 L4440 (vector) L4 Whole animal N2