The role of spermatogenesis-associated protein 6 in testicular germ cell tumors.

OBJECTIVES:To investigate the role of spermatogenesis-associated protein 6 in the testicular germ cell tumors (TGCTs). METHODS:Human embryonic carcinoma (EC)-derived cell line NTera2 was employed and randomly divided into normal control group, group, group, and duanyu1842TA6c + siduanyu1842TA6c group. The recombinant expression vector pcDNA3.1 and target sequence for siRNA was transfected into NTera2 cells in the duanyu1842TA6c group and siduanyu1842TA6c group, respectively. The protein levels in each group were determined by Western blot. Cell proliferation and apoptosis rate were assessed by 3-(4, 5-dimethylthiazol-2-yl)-2 5-diphenyl-2Htetrazolium bromide (MTT) colorimetric assay and flow cytometry (FCM) assay, respectively. In addition, Western blot was performed to investigate the expression of Bax and B-cell lymphoma (Bcl)-2 in each group. RESULTS:Compared with control group, protein levels of duanyu1842TA6 were significantly reduced in the siduanyu1842TA6c group, but were statistically increased in the duanyu1842TA6c group (P < 0.05). Similarly, the cell viability was significantly decreased by transfection with duanyu1842TA6 siRNA, but was increased by transfection with pcDNA3.1 (+)-duanyu1842TA6 compared with the control group. Moreover, the percentages of apoptosis cell were significantly higher in group than those in the three groups. After transfection of duanyu1842TA6 siRNA, the expression of Bax was significantly increased, but the expression of Bcl-2 was markedly decreased than that in the control group and duanyu1842TA6c may play an important role in TGCTs, and down-regulation of duanyu1842TA6 could lead to apoptosis of TGCTs.

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