例如:"lncRNA", "apoptosis", "WRKY"

SPARC triggers a cell-autonomous program of synapse elimination.

Proc. Natl. Acad. Sci. U.S.A.2015 Oct 27;112(43):13366-71. Epub 2015 Sep 29
Francisco J López-Murcia 1 , Beatrice Terni 1 , Artur Llobet 2
Francisco J López-Murcia 1 , Beatrice Terni 1 , Artur Llobet 2

[No authors listed]

Author information
  • 1 Laboratory of Neurobiology, Department of Pathology and Experimental Therapeutics, University of Barcelona, L'Hospitalet de Llobregat, Barcelona 08907, Spain; Bellvitge Biomedical Research Institute (IDIBELL), L'Hospitalet de Llobregat, Barcelona 08907, Spain.
  • 2 Laboratory of Neurobiology, Department of Pathology and Experimental Therapeutics, University of Barcelona, L'Hospitalet de Llobregat, Barcelona 08907, Spain; Bellvitge Biomedical Research Institute (IDIBELL), L'Hospitalet de Llobregat, Barcelona 08907, Spain allobet@ub.edu.

摘要


Elimination of the excess synaptic contacts established in the early stages of neuronal development is required to refine the function of neuronal circuits. Here we investigate whether secreted protein acidic and rich in cysteine a molecule produced by glial cells, is involved in synapse removal. production peaks when innervation of the rat superior cervical ganglion and the tail of Xenopus tropicalis tadpoles are remodeled. The formation of new cholinergic synapses in autaptic single-cell microcultures is inhibited by The effect resides in the C-terminal domain, which is also responsible for triggering a concentration- and time-dependent disassembly of stable cholinergic synapses. The loss of synaptic contacts is associated with the formation of retracted axon terminals containing multivesicular bodies and secondary lysosomes. The biological relevance of in vitro results was supported by injecting the tail of Xenopus tropicalis tadpoles with peptide 4.2, a 20-aa sequence derived from duanyu1842RC that mimics full-length protein effects. Swimming was severely impaired at ∼5 h after peptide application, caused by the massive elimination of neuromuscular junctions and pruning of axonal branches. Effects revert by 6 d after injection, as motor innervation reforms. In conclusion, duanyu1842RC triggers a cell-autonomous program of synapse elimination in cholinergic neurons that likely occurs when protein production peaks during normal development.

KEYWORDS: SPARC, Xenopus tropicalis, cholinergic synapse, neuron–glia interaction, synapse elimination

基因