Expression and significance of LRIG3 in human cervical squamous cell carcinoma.

OBJECTIVE:To explore the effect of LRIG3 in the development of cervical squamous cell carcinoma. MATERIALS AND METHODS:LRIG3 and epidermal growth factor receptor (EFGR) expressions were detected by immunohistochemistry. Western blot method was used to detect LRIG3 and EGFR protein expression at different time points. MTT, flow cytometry, and transwell chamber were adopted to examine the proliferation, apoptosis, and strength of invasion of Hela229 cell respectively. RESULTS:During the process of normal cervix deteriorating into CIN and CSCC, LRIG3 expression gradually decreased, while EGFR increased. After the transfection of LRIG3 ASODN into cells, the amount of LRIG3 expression and the cell apoptosis rate both decreased gradually, EGFR protein expression and cell proliferation rate both increased (p < 0.05), other groups remained unchanged (p > 0.05). The cell migration ability in LRIG3 ASODN transfection group was stronger than that of other groups (p < 0.05). CONCLUSION:LRIG3 plays an important role in Hela229 cell proliferation, apoptosis, and invasiveness.

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