Upregulated Expression of Karyopherin α2 is Involved in Neuronal Apoptosis Following Intracerebral Hemorrhage in Adult Rats.

Karyopherin α2 plays a central role in nucleocytoplasmic transport. It is involved in controlling the flow of genetic information and the modulation of diverse cellular activities. Here we explored the roles during the pathophysiological processes of intracerebral hemorrhage (ICH). An ICH rat model was built and evaluated according to behavioral testing. Using Western blot, immunohistochemistry, and immunofluorescence, significant upregulation of was found in neurons in brain areas surrounding the hematoma following ICH. Increasing Kduanyu15352 level was found to be accompanied by the upregulation of active caspase-3, Bax, and decreased expression of Bcl-2. Besides, Kduanyu15352 co-localized well with active caspase-3 in neurons, indicating its potential role in neuronal apoptosis. What's more, knocking down Kduanyu15352 by RNA-interference in PC12 cells reduced active caspase-3 expression. Thus, Kduanyu15352 may play a role in promoting the brain secondary damage following ICH.

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