Isoform-specific dynamic translocation of PKC by α1-adrenoceptor stimulation in live cells.

Protein kinase C plays key roles in the regulation of signal transduction and cellular function in various cell types. At least ten isoforms have been identified and intracellular localization and trafficking of these individual isoforms are important for regulation of enzyme activity and substrate specificity. duanyu1531 can be activated downstream of Gq-protein coupled receptor (GqPCR) signaling and translocate to various cellular compartments including plasma membrane (PM). Recent reports suggested that different types of GqPCRs would activate different duanyu1531 isoforms (classic, novel and atypical with different trafficking patterns. However, the knowledge of isoform-specific activation of duanyu1531 by each GqPCR is limited. α1-Adrenoceptor (α1-AR) is one of the GqPCRs highly expressed in the cardiovascular system. In this study, we examined the isoform-specific dynamic translocation of duanyu1531 in living HEK293T cells by α1-AR stimulation (α1-ARS). Rat βI, βII, δ, ε and ζ fused with GFP at C-term were co-transfected with human α1A-AR into HEK293T cells. The isoform-specific dynamic translocation of duanyu1531 in living HEK293T cells by α1-ARS using phenylephrine was measured by confocal microscopy. Before stimulation, were localized at cytosolic region. α1-ARS strongly and rapidly translocated a classical duanyu1531 duanyu1531α, (<30 s) to PM, with returning diffusively into the cytosol within 5 min. α1-ARS rapidly translocated other and to the PM (<30 s), with sustained membrane localization. One novel duanyu1531 but not another was translocated by α1-AR stimulation to the PM (<30 s) and its membrane localization was also sustained. Finally, α1-AR stimulation did not cause a diacylglycerol-insensitive atypical translocation. Our data suggest that duanyu1531α, β and ε activation may underlie physiological and pathophysiological responses of α1-AR signaling for the phosphorylation of membrane-associated substrates including ion-channel and transporter proteins in the cardiovascular system.

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