例如:"lncRNA", "apoptosis", "WRKY"

Condensin promotes the juxtaposition of DNA flanking its loading site in Bacillus subtilis.

Genes Dev.2015 Aug 1;29(15):1661-75
Xindan Wang 1 , Tung B K Le 2 , Bryan R Lajoie 3 , Job Dekker 3 , Michael T Laub 4 , David Z Rudner 1
Xindan Wang 1 , Tung B K Le 2 , Bryan R Lajoie 3 , Job Dekker 3 , Michael T Laub 4 , David Z Rudner 1
+ et al

[No authors listed]

Author information
  • 1 Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts 02115, USA;
  • 2 Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA;
  • 3 Program in Systems Biology, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA;
  • 4 Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

摘要


SMC condensin complexes play a central role in compacting and resolving replicated chromosomes in virtually all organisms, yet how they accomplish this remains elusive. In Bacillus subtilis, condensin is loaded at centromeric parS sites, where it encircles DNA and individualizes newly replicated origins. Using chromosome conformation capture and cytological assays, we show that condensin recruitment to origin-proximal parS sites is required for the juxtaposition of the two chromosome arms. Recruitment to ectopic parS sites promotes alignment of large tracks of DNA flanking these sites. Importantly, insertion of parS sites on opposing arms indicates that these "zip-up" interactions only occur between adjacent DNA segments. Collectively, our data suggest that condensin resolves replicated origins by promoting the juxtaposition of DNA flanking parS sites, drawing sister origins in on themselves and away from each other. These results are consistent with a model in which condensin encircles the DNA flanking its loading site and then slides down, tethering the two arms together. Lengthwise condensation via loop extrusion could provide a generalizable mechanism by which condensin complexes act dynamically to individualize origins in B. subtilis and, when loaded along eukaryotic chromosomes, resolve them during mitosis.

KEYWORDS: DNA segregation, Hi-C, ParB, SMC, lengthwise condensation, mitosis, parS

基因


原始数据


 保存测序数据
Sample name
Organism Experiment title Sample type Library instrument Attributes
development stagegenotyperestriction enzymesource name
41_Rudnerlab_ChIPseq_BWX3209
Bacillus subtilis PY79 GSM1821870: 41_Rudnerlab_ChIPseq_BWX3209; Bacillus subtilis PY79; ChIP-Seq ChIP-Seq Illumina MiSeq CH, 37C delta-parB::spec, pelB::Psoj mcherry-parB1(Vc) (cat) Bacillus subtilis PY79, ?parB::spec, pelB::Psoj mcherry-parB1(Vc) (cat)
40_Rudnerlab_ChIPseq_BWX3208
Bacillus subtilis PY79 GSM1821869: 40_Rudnerlab_ChIPseq_BWX3208; Bacillus subtilis PY79; ChIP-Seq ChIP-Seq Illumina MiSeq CH, 37C delta-parB::spec, pelB::Psoj mcherry-parB(delta-parS) (cat) Bacillus subtilis PY79, ?parB::spec, pelB::Psoj mcherry-parB(?parS) (cat)
39_Rudnerlab_HindIII_HiC_BWX3359_42C40min
Bacillus subtilis PY79 GSM1821868: 39_Rudnerlab_HindIII_HiC_BWX3359_42C40min; Bacillus subtilis PY79; OTHER OTHER Illumina HiSeq 2500 CH, 30C to 42C, 40min parS-delta-9, -94degrees parS no ab, smcts02 spec HindIII Bacillus subtilis PY79, parS?9, -94°parS no ab, smcts02 spec
38_Rudnerlab_HindIII_HiC_BWX3359_30C
Bacillus subtilis PY79 GSM1821867: 38_Rudnerlab_HindIII_HiC_BWX3359_30C; Bacillus subtilis PY79; OTHER OTHER Illumina HiSeq 2500 CH, 30C parS-delta-9, -94degrees parS no ab, smcts02 spec HindIII Bacillus subtilis PY79, parS?9, -94°parS no ab, smcts02 spec
37_Rudnerlab_HindIII_HiC_BWX3304_42C20min
Bacillus subtilis PY79 GSM1821866: 37_Rudnerlab_HindIII_HiC_BWX3304_42C20min; Bacillus subtilis PY79; OTHER OTHER Illumina HiSeq 2500 CH, 30C to 42C, 20min parS-delta-8, smcts02 spec HindIII Bacillus subtilis PY79, parS?8, smcts02 spec