[No authors listed]
Hepatomaâderived growth factorâ2 (HDGFâ2) is expressed in neurons, astrocytes and oligodendrocytes of the adult mouse brain. However, it has remained elusive whether HDGFâ2 is expressed in the spinal cord and is involved in the its development and repair. In the present study, the expression of HDGFâ2 was investigated in rat spinal cords at different developmental stages and following spinal cord injury (SCI). Protein levels of HDGFâ2 were examined using western blot analysis, while the distribution pattern and cell populations of HDGFâ2 protein expression were characterized using immunohistochemistry. Western blot analysis demonstrated that the levels of HDGFâ2 protein expression were the greatest in the spinal cord on embryonic day 19, and were also highly expressed in rat spinal cords on postânatal day 7 (P7); however, they were low at P14 and not detectable at two months. HDGFâ2 expression was significantly upregulated in the embryonic spinal cord and injured spinal cord. By contrast, the expression of HDGFâ2 was low in uninjured adult spinal cords. HDGFâ2 expression in the fetal rat spinal cord and injured spinal cord was significantly higher than that in uninjured adult spinal cord tissues (P<0.05). The number of cells positive for HDGFâ2 was 141±62, 107±33 and 92±18 at days 1, 21 and 45 following SCI, respectively, as opposed to 50±9 in uninjured rats, and a significant difference was identified between the different timeâpoints following SCI (P<0.01). In conclusion, the overexpression of HDGFâ2 in the embryonic spinal cord and injured spinal cord may be involved in fetal spinal cord development and repair of SCI, respectively.
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