LARP4B is an AU-rich sequence associated factor that promotes mRNA accumulation and translation.

mRNAs are key molecules in gene expression and subject to diverse regulatory events. Regulation is accomplished by distinct sets of trans-acting factors that interact with mRNAs and form defined mRNA-protein complexes (mRNPs). The resulting "mRNP code" determines the fate of any given mRNA and thus controlling gene expression at the post-transcriptional level. The La-related protein 4B belongs to an evolutionarily conserved family of RNA-binding proteins characterized by the presence of a La-module implicated in direct RNA binding. Biochemical experiments have shown previously direct interactions of with factors of the translation machinery. This finding along with the observation of an association with actively translating ribosomes suggested that Lduanyu374B is a factor contributing to the mRNP code. To gain insight into the function of Lduanyu374B in vivo we tested its mRNA association at the transcriptome level and its impact on the proteome. PAR-CLIP analyses allowed us to identify the in vivo RNA targets of We show that Lduanyu374B binds to a distinct set of cellular mRNAs by contacting their Biocomputational analysis combined with in vitro binding assays identified the motif on mRNA targets. The reduction of cellular Lduanyu374B levels leads to a marked destabilization of its mRNA targets and consequently their reduced translation. Our data identify Lduanyu374B as a component of the mRNP code that influences the expression of its mRNA targets by affecting their stability.

KEYWORDS: {{ getKeywords(articleDetailText.words) }}