例如:"lncRNA", "apoptosis", "WRKY"

Ku stabilizes replication forks in the absence of Brc1.

PLoS ONE. 2015 May 12;10(5):e0126598. eCollection 2015
Arancha Sánchez 1 , Paul Russell 1
Arancha Sánchez 1 , Paul Russell 1

[No authors listed]

Author information
  • 1 Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, California, United States of America.

摘要


DNA replication errors are a major source of genome instability in all organisms. In the fission yeast Schizosaccharomyces pombe, the DNA damage response protein Brc1 binds phospho-histone H2A (γH2A)-marked chromatin during S-phase, but how Brc1 protects genome integrity remains unclear. Here we report that the non-homologous end-joining (NHEJ) protein Ku becomes critical for survival of replication stress in brc1∆ cells. Ku's protective activity in brc1∆ cells does not involve its canonical NHEJ function or its roles in protecting telomeres or shielding DNA ends from Exo1 exonuclease. In brc1∆ pku80∆ cells, nuclear foci of Rad52 homologous recombination (HR) protein increase and Mus81-Eme1 Holliday junction resolvase becomes critical, indicating increased replication fork instability. Ku's localization at a ribosomal DNA replication fork barrier associated with frequent replisome-transcriptosome collisions increases in brc1∆ cells and increased collisions correlate with an enhanced requirement for Brc1. These data indicate that Ku stabilizes replication forks in the absence of Brc1.