例如:"lncRNA", "apoptosis", "WRKY"

The basal transcription complex component TAF3 transduces changes in nuclear phosphoinositides into transcriptional output.

Mol. Cell. 2015 May 7;58(3):453-67. Epub 2015 Apr 09
Yvette Stijf-Bultsma 1 , Lilly Sommer 2 , Maria Tauber 3 , Mai Baalbaki 4 , Panagiota Giardoglou 5 , David R Jones 2 , Kathy A Gelato 3 , Jason van Pelt 5 , Zahid Shah 6 , Homa Rahnamoun 7 , Clara Toma 7 , Karen E Anderson 8 , Philip Hawkins 8 , Shannon M Lauberth 7 , Anna-Pavlina G Haramis 5 , Daniel Hart 4 , Wolfgang Fischle 3 , Nullin Divecha 9
Yvette Stijf-Bultsma 1 , Lilly Sommer 2 , Maria Tauber 3 , Mai Baalbaki 4 , Panagiota Giardoglou 5 , David R Jones 2 , Kathy A Gelato 3 , Jason van Pelt 5 , Zahid Shah 6 , Homa Rahnamoun 7 , Clara Toma 7 , Karen E Anderson 8 , Philip Hawkins 8 , Shannon M Lauberth 7 , Anna-Pavlina G Haramis 5 , Daniel Hart 4 , Wolfgang Fischle 3 , Nullin Divecha 9
+ et al

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Author information
  • 1 The Inositide Laboratory, Centre for Biological Sciences, Highfield Campus, University of Southampton, Southampton SO171BJ, UK; The Inositide Laboratory, the CRUK Manchester Institute, the University of Manchester, Wilmslow Road, Manchester M204BX, UK.
  • 2 The Inositide Laboratory, the CRUK Manchester Institute, the University of Manchester, Wilmslow Road, Manchester M204BX, UK.
  • 3 Laboratory of Chromatin Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
  • 4 University of California, San Francisco, Mail Code 3120, Smith Cardiovascular Research Building, 555 Mission Bay Boulevard, South San Francisco, CA 94158-9001, USA.
  • 5 Institute of Biology (IBL), Leiden University, Sylvius Laboratory, Sylviusweg 72, 2333 BE Leiden, the Netherlands.
  • 6 The Inositide Laboratory, Centre for Biological Sciences, Highfield Campus, University of Southampton, Southampton SO171BJ, UK.
  • 7 Division of Biological Sciences, Department of Molecular Biology, University of California, San Diego, La Jolla, CA 92093, USA.
  • 8 Signaling Laboratory, The Babraham Institute, Cambridge, Cambridgeshire CB22 3AT, UK.
  • 9 The Inositide Laboratory, Centre for Biological Sciences, Highfield Campus, University of Southampton, Southampton SO171BJ, UK; The Inositide Laboratory, the CRUK Manchester Institute, the University of Manchester, Wilmslow Road, Manchester M204BX, UK. Electronic address: n.divecha@soton.ac.uk.
全文

摘要


Phosphoinositides (PI) are important signaling molecules in the nucleus that influence gene expression. However, if and how nuclear PI directly affects the transcriptional machinery is not known. We report that the lipid kinase PIP4K2B regulates nuclear PI5P and the expression of myogenic genes during myoblast differentiation. A targeted screen for PI interactors identified the PHD finger of TAF3, a TATA box binding protein-associated factor with important roles in transcription regulation, pluripotency, and differentiation. We show that the PI interaction site is distinct from the known H3K4me3 binding region of TAF3 and that PI binding modulates association of TAF3 with H3K4me3 in vitro and with chromatin in vivo. Analysis of TAF3 mutants indicates that TAF3 transduces PIP4K2B-mediated alterations in PI into changes in specific gene transcription. Our study reveals TAF3 as a direct target of nuclear PI and further illustrates the importance of basal transcription components as signal transducers.

基因