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Response of Dictyostelium discoideum to UV-C and involvement of poly (ADP-ribose) polymerase.

Cell Prolif.2015 Jun;48(3):363-74. doi:10.1111/cpr.12182. Epub 2015 Apr 08
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摘要


OBJECTIVES:Radiation and chemical mutagens are direct DNA-damaging agents and ultraviolet (UV) radiation is frequently used in biological studies. Consequent to ozone depletion, UV-C could become a great challenge to living organisms on earth, in the near future. The present study has focused on the role of poly (ADP-ribose) polymerase during UV-C-induced growth and developmental changes in Dictyostelium discoideum, a phylogenetically important unicellular eukaryote. MATERIALS AND METHODS:Dictyostelium discoideum cells were exposed to different doses of UV-C and activity, and effects of its inhibition were studied. Expression of developmentally regulated genes yakA, car1, aca, csA, regA, ctnA, ctnB, gp24, hspD and dsn were analysed using semiquantitative RT-PCR. RESULTS:We report that the D. discoideum cells displayed Pduanyu37 activation within 2 min of UV-C irradiation and there was increase in NO levels in a dose-dependent manner. UV-C-irradiated cells had impaired growth, delayed or blocked development and delayed germination compared to control cells. In our previous studies we have shown that inhibition of Pduanyu37 recovered oxidative stress-induced changes in D. discoideum; however, intriguingly Pduanyu37 inhibition did not correct all defects as effectively in UV-C-irradiated cells. This possibly was due to interplay with increased NO signalling. CONCLUSIONS:Our results signify that UV-C and oxidative stress affected growth and development in D. discoideum by different mechanisms; these studies could provide major clues to complex mechanisms of growth and development in higher organisms.

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