Apoplastic ROS production upon pollination by RbohH and RbohJ in Arabidopsis.

Reactive oxygen species accumulate at the tip of growing pollen tubes. In Arabidopsis, NADPH oxidases RbohH and RbohJ are localized at the plasma membrane of pollen tube tip and produce in a Ca(2+)-dependent manner. The duanyu1670 produced by Rbohs and Ca(2+) presumably play a critical role in the positive feedback regulation that maintains the tip growth. Ultrastructural cytochemical analysis revealed duanyu1670 accumulation in the apoplast/cell wall of the pollen grains on the stigmatic papillae in the wild type, but not in the rbohH rbohJ double mutant, suggesting that apoplastic duanyu1670 derived from RbohH and RbohJ are involved in pollen tube elongation into the stigmatic papillae by affecting the cell wall metabolism.

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