[No authors listed]
Bacillus subtilis Ï(D) is an alternative Ï factor that possesses a core-independent promoter -10 element binding specificity despite the lack of a distinct footprint on its cognate promoter. We wished to determine whether this property is common to alternative Ï factors. To this end, we over-expressed B. subtilis Ï(B) in Escherichia coli and analyzed its DNA binding ability by electrophoretic mobility shift assay and DNase I footprinting. The major complex formed by Ï(B) and its cognate promoter DNA is heparin-sensitive. However, in contrast to the -10 element binding specificity observed for B. subtilis Ï(D) , the promoter binding of Ï(B) is specific for the -35 element. These and other results clearly demonstrate that alternative Ï factors possess different promoter-binding characteristics, and make core-independent contributions to recognition of their cognate promoters.
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