例如:"lncRNA", "apoptosis", "WRKY"

Drug resistance effects of ribosomal protein L24 overexpression in hepatocellular carcinoma HepG2 cells.

Asian Pac. J. Cancer Prev.2014;15(22):9853-7. doi:10.7314/apjcp.2014.15.22.9853
Yong-Li Guo 1 , Qing-Sheng Kong , Hong-Sheng Liu , Wen-Bin Tan
Yong-Li Guo 1 , Qing-Sheng Kong , Hong-Sheng Liu , Wen-Bin Tan

[No authors listed]

Author information
  • 1 Oncology Department of the Jining First People's Hospital, Jining, Shandong, People's Republic of China E-mail : 1392144@163.com.

摘要


BACKGROUND:The morbidity and mortality rate of liver cancer continues to rise in China and advanced cases respond poorly to chemotherapy. Ribosomal protein L24 has been reported to be a potential therapeutic target whose depletion or acetylation inhibits polysome assembly and cell growth of cancer. MATERIALS AND METHODS:Total RNA of cultured amycin-resistant and susceptible HepG2 cells was isolated, and real time quantitative RT-PCR were used to indicate differences between amycin-resistant and susceptible strains of HepG2 cells. Viability assays were used to determine amycin resistance in RPL24 transfected and control vector and null- transfected HepG2 cell lines. RESULTS:The ribosomal protein L24 transcription level was 7.7 times higher in the drug-resistant HepG2 cells as compared to susceptible cells on quantitative RT-PCR analysis. This was associated with enhanced drug resistance as determined by methyl tritiated thymidine (3H-TdR) incorporation. CONCLUSIONS:The ribosomal protein L24 gene may have effects on drug resistance mechanisms in hepatocellular carcinoma HepG2 cells.