例如:"lncRNA", "apoptosis", "WRKY"

A novel open-barrel structure of octameric translin reveals a potential RNA entryway.

J. Mol. Biol.2015 Feb 27;427(4):756-62. Epub 2014 Nov 26
Elad Eliahoo 1 , Ailie Marx 1 , Haim Manor 1 , Akram Alian 2
Elad Eliahoo 1 , Ailie Marx 1 , Haim Manor 1 , Akram Alian 2

[No authors listed]

Author information
  • 1 Department of Biology, Technion-Israel Institute of Technology, Haifa 320003, Israel.
  • 2 Department of Biology, Technion-Israel Institute of Technology, Haifa 320003, Israel. Electronic address: alian@tx.technion.ac.il.

摘要


The single-stranded DNA (ssDNA)/RNA binding protein translin was suggested to be involved in chromosomal translocations, telomere metabolism, and mRNA transport and translation. Oligonucleotide binding surfaces map within a closed cavity of translin octameric barrels, raising the question as to how DNA/RNA gain access to this inner cavity, particularly given that, to date, none of the barrel structures reported hint to an entryway. Here, we argue against a mechanism by which translin octamers may "dissociate and reassemble" upon RNA binding and report a novel "open"-barrel structure of human translin revealing a feasible DNA/RNA entryway into the cavity. Additionally, we report that translin not only is confined to binding of ssDNA oligonucleotides, or single-stranded extensions of double-stranded DNA (dsDNA), but also can bind single-stranded sequences internally embedded in dsDNA molecules.

KEYWORDS: RNA, TRAX, octamer, open barrel, translin

基因